flavin reductase (NADPH)

This is an abbreviated version!
For detailed information about flavin reductase (NADPH), go to the full flat file.

Word Map on EC


reduced riboflavin


alkanesulfonate FMN reductase, ArsH, azoreductase, AZRü, Biliverdin-IX beta-reductase, CysJ, flavin oxidoreductase, flavin reductase, flavin reductase 1, flavin reductase Nr1, flavin reductase P, FLR, FR1, Frb, Frd188, frd2, fre, Fre oxidoreductase, Fre-1, FRG/FRaseI, FRGvf, FRP, FRPvh, GHBP, Green heme binding protein, HpaC, ipa-43d, More, NAD(P)H-dependent H2O2-forming flavin reductase, NADPH-dependant FMN reductase, NADPH-dependent diaphorase, NADPH-flavin oxidoreductase, NADPH-flavin reductase, NADPH-FMN oxidoreductase, NADPH-FMN reductase, NADPH-nitrofurazone reductase, NADPH-specific flavin reductase, NADPH:FAD oxidoreductase, NADPH:flavin oxidoreductase, NADPH:FMN oxidoreductase, NfrA1, nitro/flavin reductase, non-luminescent-bacterial NfsA/Frp-type enzyme, SsuE, TVAG_517010


     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
       flavin reductase (NADPH)


Cloned on EC - flavin reductase (NADPH)

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CLONED (Commentary)
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expression of wild-type enzyme and mutant R203A in Escherichia coli
expression of wild-type enzyme in Escherichia coli
frb gene inserted into plasmid pET21-a, introduced into Escherichia coli BL21 (DE3), cultivated at 30°C
gene frd2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene fre-1, DNA and amino acid sequence determination and analysis, transcribed as a bicistronic pre-mRNA together with gene dcs-1, encoding Hint-related 7meGMP-directed hydrolase DCS-1
gene frp, functional expression of the enzyme fused to yellow fluorescence protein YFP in a Vibrio harveyi strain lacking the frp gene, subcloning and expression in Escherichia coli strains JM109 and BL21(DE3), the yellow fluorescence protein YFP is a mutant of the green fluorescence protein GFP, with mutations S65G, V68L, S72A, and T203Y
gene ipa-43d, DNA and amino acid sequence determination and analysis, genetic structure and sequence comparison, recombinant overexpression in Escherichia coli J83
gene TVAG_517010, DNA and amino acid sequence determination and analysis, quantitative RT-PCR expression analysis of FR1 in metronidazole-susceptible isolates C1, G3, JH31A and Tv2 , re-introduction of FR1 into an aerobically-resistant strain, transfection of the highly metronidazole-resistant isolate B7268 with the FR1 gene restores metronidazole susceptibility
PCR-amplification, expression in Escherichia coli Bl21 (deltaDE3)