1.5.1.19: D-nopaline dehydrogenase
This is an abbreviated version!
For detailed information about D-nopaline dehydrogenase, go to the full flat file.
Word Map on EC 1.5.1.19
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1.5.1.19
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cauliflower
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agrobacterium
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tumefaciens
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kanamycin
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nptii
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camv35s
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octopine
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potyvirus
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xanthi
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construct-specific
- 1.5.1.19
- cauliflower
- agrobacterium
- tumefaciens
- kanamycin
- nptii
-
camv35s
- octopine
- potyvirus
-
xanthi
-
construct-specific
Reaction
Synonyms
Atu6015, D-nopaline synthase, dehydrogenase, nopaline, flavin-containing opine dehydrogenase, GN325_22415, nopaline dehydrogenase, nopaline synthase, NOS
ECTree
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General Information
General Information on EC 1.5.1.19 - D-nopaline dehydrogenase
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additional information
crown gall disease caused by Allorhizobium vitis is one of the most destructive diseases in grapevine cultivation from an economical perspective. The bacterial pathogen exists systemically in grapevines and spreads through cuttings used for propagation material obtained from grapevines, living vines, and mother blocks, which poses a high risk for certified grapevine nursery material cultivation, nursery industry, and growers. Therefore, in this study, real-time PCR-based methods were developed for effectively and accurately determining the presence and the density of the bacterial pathogen in grapevine nursery material and the data used for indexing programs. Octopine-, nopaline-, and vitopine-catabolizing A. vitis strain-specific primer and locked nucleic acid (LNA) probe sets were determined depending on the ocs, nos, vis, vitopine iaaM, and vitopine virD2 genes. Primer-probe sets were then used to amplify the octopine synthase gene (62 bp) from octopine strains, the nopaline synthase gene (78 bp) from nopaline strains, and the vitopine synthase gene (60 bp) from vitopine strains, as well as the vitopine iaaM gene (60 bp) and the vitopine virD2 gene (66 bp) of A. vitis. The detection of Allorhizobium vitis strains from bacterial suspension or extracted plant sap can be achieved within a short time (i.e. 20-25 min) with real-time PCR-based assays using the designed primer and probe sets, method development and evaluation, overview
additional information
Agrobacterium vitis ICMP 10753
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crown gall disease caused by Allorhizobium vitis is one of the most destructive diseases in grapevine cultivation from an economical perspective. The bacterial pathogen exists systemically in grapevines and spreads through cuttings used for propagation material obtained from grapevines, living vines, and mother blocks, which poses a high risk for certified grapevine nursery material cultivation, nursery industry, and growers. Therefore, in this study, real-time PCR-based methods were developed for effectively and accurately determining the presence and the density of the bacterial pathogen in grapevine nursery material and the data used for indexing programs. Octopine-, nopaline-, and vitopine-catabolizing A. vitis strain-specific primer and locked nucleic acid (LNA) probe sets were determined depending on the ocs, nos, vis, vitopine iaaM, and vitopine virD2 genes. Primer-probe sets were then used to amplify the octopine synthase gene (62 bp) from octopine strains, the nopaline synthase gene (78 bp) from nopaline strains, and the vitopine synthase gene (60 bp) from vitopine strains, as well as the vitopine iaaM gene (60 bp) and the vitopine virD2 gene (66 bp) of A. vitis. The detection of Allorhizobium vitis strains from bacterial suspension or extracted plant sap can be achieved within a short time (i.e. 20-25 min) with real-time PCR-based assays using the designed primer and probe sets, method development and evaluation, overview
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