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0.04
5'-phospho-pyridoxyl-3-alanine
-
-
0.1
5'-phospho-pyridoxyl-3-aminobenzoate
-
-
0.04
5'-phospho-pyridoxyl-4-aminobutyrate
-
-
0.035
5'-phospho-pyridoxyl-5-aminovalerate
-
-
0.01
5'-phospho-pyridoxyl-aniline
-
-
0.1
5'-phospho-pyridoxyl-p-aminonitrobenzene
-
-
0.004
5'-phospho-pyridoxyl-p-aminophenol
-
-
33
pyridoxal 5'-phosphate
-
recombinant enzyme after cleavage from fusion protein
0.009 - 28
pyridoxamine 5'-phosphate
0.0002 - 0.76
pyridoxine 5'-phosphate
additional information
additional information
-
turnover numbers of mutant enzymes H199A, H199N, R197E, R197M, Y17F, R14E, R14M
-
0.009
pyridoxamine 5'-phosphate
-
-
0.103
pyridoxamine 5'-phosphate
-
-
0.103
pyridoxamine 5'-phosphate
-
-
0.2
pyridoxamine 5'-phosphate
-
pH 7.6, 37°C
1.72
pyridoxamine 5'-phosphate
-
recombinant enzyme
28
pyridoxamine 5'-phosphate
-
recombinant enzyme after cleavage from fusion protein
0.0002
pyridoxine 5'-phosphate
-
mutant enzyme K72P, at pH 8.0 and 37°C
0.0004
pyridoxine 5'-phosphate
-
mutant enzyme K72A, at pH 8.0 and 37°C
0.0015
pyridoxine 5'-phosphate
-
mutant enzyme K72Q, at pH 8.0 and 37°C
0.002
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R225H
0.008
pyridoxine 5'-phosphate
R197M mutant enzyme
0.01
pyridoxine 5'-phosphate
-
in 25 mM potassium phosphate, pH 7.8 and 25°C
0.012
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R116Q/R225H
0.019
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R141C
0.022
pyridoxine 5'-phosphate
-
wild type enzyme, at pH 8.0 and 37°C
0.022
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme G118R
0.024
pyridoxine 5'-phosphate
-
mutant enzyme K72R, at pH 8.0 and 37°C
0.03
pyridoxine 5'-phosphate
H199N mutant enzyme
0.03
pyridoxine 5'-phosphate
R197M mutant enzyme
0.037
pyridoxine 5'-phosphate
mutant enzyme R95C, at pH 8.0 and 37°C
0.037
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R116Q
0.037
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R95C
0.04
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R229W
0.05
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme N84W/K145A/F177A
0.05
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R215L
0.05
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L/R215L
0.057
pyridoxine 5'-phosphate
pH 7.6, 37°C, wild-type enzyme, HEPES buffer
0.06
pyridoxine 5'-phosphate
D49A mutant enzyme
0.062
pyridoxine 5'-phosphate
pH 7.6, 37°C, wild-type enzyme, TRIS buffer
0.07
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme N84A/K145A/F177A
0.09
pyridoxine 5'-phosphate
-
-
0.09
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L
0.09
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L/R24L/R215L
0.13
pyridoxine 5'-phosphate
-
0.13
pyridoxine 5'-phosphate
-
pH 8.5, 37°C
0.14
pyridoxine 5'-phosphate
H199A mutant enzyme
0.14
pyridoxine 5'-phosphate
R14M mutant enzyme
0.16
pyridoxine 5'-phosphate
R14E mutant enzyme
0.17
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme K145A/F177A
0.18
pyridoxine 5'-phosphate
wild type enzyme, at pH 8.0 and 37°C
0.19
pyridoxine 5'-phosphate
-
pH 7.6, 37°C
0.19
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L-R24L
0.2
pyridoxine 5'-phosphate
-
recombinant enzyme, in phosphate and HEPES buffer at pH 7.6
0.25
pyridoxine 5'-phosphate
pH 7.6, 37°C, wild-type enzyme
0.28
pyridoxine 5'-phosphate
-
37°C, pH 7.6
0.3
pyridoxine 5'-phosphate
-
recombinant enzyme, in Tris buffer at pH 7.6
0.6
pyridoxine 5'-phosphate
Y17F mutant enzyme
0.7
pyridoxine 5'-phosphate
-
-
0.7
pyridoxine 5'-phosphate
-
-
0.76
pyridoxine 5'-phosphate
-
recombinant enzyme