1.4.3.5: pyridoxal 5'-phosphate synthase
This is an abbreviated version!
For detailed information about pyridoxal 5'-phosphate synthase, go to the full flat file.
Reaction
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Synonyms
ePNPOx, FprA protein, oxidase, pyridoxamine phosphate, Pdx1, PDX3, PdxH, PMP oxidase, PNP oxidase, PNP/PMP oxidase, PNPO, PNPOx, PPOX, pyridox(am)ine 5'-phosphate oxidase, pyridoxal 5'-phosphate synthase, pyridoxal 5'-phosphate synthetic enzyme, pyridoxamine (pyridoxine) 5'-phosphate oxidase, pyridoxamine (pyridoxine) 5’-phosphate:O2 oxidoreductase (deaminating), pyridoxamine 5'-phosphate oxidase, pyridoxamine phosphate oxidase, pyridoxamine-5-phosphate oxidase, pyridoxamine-phosphate oxidase, pyridoxaminephosphate oxidase (EC 1.4.3.5: deaminating), pyridoxinamine 5'-phosphate oxidase, pyridoxine (pyridoxamine) 5 '-phosphate oxidase, pyridoxine (pyridoxamine) 5'-phosphate oxidase, pyridoxine (pyridoxamine) phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine 5'-phosphate oxidase`, pyridoxine 5-phosphate oxidase, pyridoxine phosphate oxidase, pyridoxine-5'-phosphate oxidase, pyridoxine/pyridoxamine 5'-phosphate oxidase, pyridoxine/pyridoxamine phosphate oxidase, sgll, Sgll/PNPO, Ylr456w, Ypr172w
ECTree
Temperature Stability
Temperature Stability on EC 1.4.3.5 - pyridoxal 5'-phosphate synthase
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24
-
pH 7.0, immobilized enzyme: 50% loss of activity after 10 days, free enzyme: 50% loss of activity after 2-3 days
25
-
half-life: 4 days immobilized enzyme, less than 1 day free enzyme
44.3
Tm-value in presence of FMN, mutant enzyme G118R
45
-
above 45°C, loss of activity after 30 min
48.2
Tm-value in presence of FMN, mutant enzyme R116Q
48.7
Tm-value in presence of FMN, mutant enzyme G118R
49.2
Tm-value in presence of FMN, mutant enzyme R141C
5
-
pH 7.0, immobilized enzyme stable for 25 days, free enzyme: rapid loss of activity
52.3
Tm-value in presence of FMN, mutant enzyme R141C
52.7
Tm-value in presence of FMN, mutant enzyme X262Q
53.7
Tm-value in presence of FMN, mutant enzyme R116Q/R225H
55.4
Tm-value in presence of FMN, wild-type enzyme
55.9
Tm-value in presence of FMN, mutant enzyme X262Q
58.3
Tm-value in presence of FMN, wild-type enzyme
60.5
Tm-value in presence of FMN, mutant enzyme R225H
62.1
Tm-value in presence of FMN, mutant enzyme R225H
65
-
no loss in activity after incubation at temperatures below 50°C, 30% to 40% activity loss after 15 min at 65°C
66.6
Tm-value, mutant enzyme N84W/K145A/F177A
66.7
Tm-value, mutant enzyme N84A/K145A/F177A
68
Tm-value, mutant enzyme R23L/R24L/R215L
68.9
Tm-value, mutant enzyme R23L/R215L
69
Tm-value, mutant enzyme R215L
70.9
Tm-value, mutant enzyme K145A/F177A
71.1
Tm-value, mutant enzyme R23L/R24L
50.1
Tm-value in presence of FMN, mutant enzyme R116Q
50.1
Tm-value in presence of FMN, mutant enzyme R116Q/R225H
70
Tm-value, wild-type enzyme
70
Tm-value, mutant enzyme R23L