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1.4.3.2: L-amino-acid oxidase

This is an abbreviated version!
For detailed information about L-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.2

Reaction

an L-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

AAD, ACTX-6, ACTX-8, Akbu-LAAO, APIT, apotxin, aromatic L-amino acid oxidase, Balt-LAAO-I, bordonein-L, Bpir-LAOO-1, CC-LPOX, DAA, Dolabellanin, escapin, head kidney and gill, IL4I1, Il4i1 protein, ink toxin 1, Interleukin Four Induced Gene 1 protein, L-AAO, L-AAO/MOG, L-AAO1, L-amino acid oxidase, L-amino acid: O2 oxidoreductase, L-amino acid:O2 oxidoreductase, L-amino acid:O2 oxidoreductase (deaminating), L-aminooxidase, L-Aox, L-phenylalanine oxidase, LAAO, LAAO-1, LAAO-I, LAAO-II, LAAO1, LAAO4, LAAOI, LAAOII, LAO, LAO1, M-LAO, ophio-amino-acid oxidase, Pm1, RoLAAO, sarA, Sebastes schlegeli antibacterial protein, Sebastes schlegelii antibacterial protein, SSAP, TJ-LAO, toxophallin, TSV-LAO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.2 L-amino-acid oxidase

Purification

Purification on EC 1.4.3.2 - L-amino-acid oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE-cellulose column chromatography, and Sephadex G200 gel filtration
-
ammonium sulfate precipitation, Mono Q column chromatography, and heparin-Sepharose column chromatography
-
amylose column chromatography and Ni-NTA column chromatography
-
benzamidine-Sepharose 4 column chromatography, phenyl-Sepharose 6 column chromatography, and Sephacryl S-100 gel filtration
-
benzamidine-Sepharose and phenyl-Sepharose column chromatography
-
by cation ion exchange chromatography, gel filtration and anion ion exchange chromatography, to homogeneity
-
by gel filtration
-
by gel filtration followed by anion exchange chromatography
-
by gel filtration followed by ion-exchange chromatography, 51.3fold
-
by gel filtration, affinity and hydrophobic interaction chromatography
by gel filtration, ion exchange chromatography, 100fold purified
-
by gel filtration, ion-exchange and heparin chromatography, to homogeneity
by HPLC and sequence grade
-
by immobilized metal ion affinity chromatography
-
by ion exchange and gel filtration, to homogeneity
-
by ion-exchange chromatography and gel filtration, 12.9fold
-
by molecular exclusion, ion exchange and hydrophobic chromatography
by Sephadex G-75 gel filtration, Sephadex G-25 desalting, Q ion-exchange chromatography
by size exclusion chromatography
-
by two chromatographic steps on Sephadex G-100 and CM Sephadex C-50
CM-Sepharose column chromatography, phenyl-Sepharose CL-4B column chromatography, benzamidine Sepharose and C18 RP column chromatography
-
DEAE cellulose column chromatography and Sephadex G-100 gel filtration
-
DEAE column chromatography and Sephacryl-S200 gel filtration
-
DEAE Sephadex A-50 column chromatography, and Sephadex G-75 gel filtration
-
DEAE Sepharose column chromatography, and Superdex 200 gel filtration
DEAE Sepharose F column chromatography and Source 30 column chromatography
-
DEAE-Toyopearl column chromatography, phenyl Toyopearl column chromatography, and L-lysine-Toyopearl resin column chromatography
-
G-75 gel filtration, HPLC-Shodex ES-502N 7C column chromatography, and Lentil lectin column chromatography
-
gel filtration
gel filtration followed by ion exchange chromatography, to homogeneity
-
HiPrep phenyl column chromatography and Ni-NTA agarose column chromatography
HPLC AP1 column chromatography and Superdex 75 gel filtration
-
ion-exchange column chromatography, Sephadex G-75 gel filtration and C4 reverse phase protein pack SP 5PW chromatography
-
Mono Q and heparin column chromatography
-
Mono Q column chromatography and Sephacryl S-100 gel filtration
-
Mono Q column chromatography and Sephacryl S-100 gel filtration or phenyl Sepharose column chromatography, Affi-gel column chromatography, and Sephacryl S-200 gel filtration
-
native enzyme 54fold from venom by gel filtration and ultrafiltration, anion exchange chromatography, and lectin affinity chromatography to homogeneity
-
native enzyme 700fold from serum by concanavalin A lectin affinity chromatography, anion exchange HPLC, hydroxyapatite HPLC and gel filtration HPLC
-
native enzyme by anion exchange chromatography, gel filtration, and heparin affinity chromatography to homogeneity
native enzyme by gel filtration, cation and anion exchange chromatography, and hydroxyapatite chromatography
native enzyme from venom by anion exchange and heparin affinity chromatography
-
native enzyme from venom by cation exchange and hydrophobic interaction chromatography, followed by adosrption chromatography and HPLC
native enzyme from venom by gel filtration, anion exchange and heparin affinity chromatography
-
native enzyme from venom by two repeated steps of anion exchange chromatography
-
native enzyme to high molecular homogeneity by ion exchange chromatography and reverse phase chromatography, followed by anion exchange chromatgography, ultrafiltration, and gel filtration
-
native extracellular enzyme 11.2fold from venom by anion exchange chromatography and 3 steps of gel filtration, one of which is HPLC
-
native extracellular enzyme from venom by gel filtration and anion exchange chromatography
-
native isozymes by lectin affinity and anion exchange chromatography, isozyme MPLAO1 and MPLAO3 are purified to homogeneity, while isozyme MPLAO2 is not
native isozymes from venom by 2times anion exchange chromatography and dialysis to homogeneity
-
Ni-NTA column chromatography and Superdex 200 gel filtration
phenyl Sepharose column chromatography and Sephadex G-75 gel filtration
-
phenyl Toyopearl column chromatography, DEAE Toyopearl column chromatography, aminooctyl Toyopearl column chromatography and hydroxyapatite column chromatography
-
Protein Pack SP 5PW anion exchange chromatography and Superdex 200 gel filtration
-
purification by cationic exchange, size exclusion, and glycoprotein affinity
purification by molecular exclusion, ion exchange and affinity purification
recombinant enzyme
-
recombinant His-tagged enzyme 35fold from Escherichia coli strain Rosetta (DE3) by nickel affinity and hydrophbic interaction chromatography
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography, native extracellular enzyme from Platichthys stellatus-derived epidermal mucus by ultracentrifugation, hydrophobic interaction chromatography, and gel filtration
Resource Q column chromatography, HiTrap Heparin column chromatograph, yand Sephadex G-75 gel filtration
-
Sephacryl S-200 gel filtration, Sephacryl S-300 gel filtration, and DEAE Sepharose CL-6B column chromatography
-
Sephacryl S200 column chromatography
-
Sephacryl S200 gel filtration and MonoQ column chromatography
-
Sephadex G-100 gel filtration, DEAE-cellulose column chromatography, and phenyl-agarose column chromatography
-
Sephadex G-100 gel filtration, HPS-7 column chromatography, DEAE-cellulose DE52 column chromatography, and CM-cellulose CM52 column chromatography
-
Sephadex G-100 gel filtration, Q column chromatography, and HiTrap heparin column chromatography
-
Sephadex G-50 SF gel filtration, CM-cellulose CM52 column chromatography, and HPS-7 column chromatography
-
Sepharose-IDA column chromatography, phenyl-Sepharose column chromatography, and Sephadex G-100 gel filtration
-
Source 15Q column chromatography and Superose 12 gel filtration
-
SP-Sepharose column chromatography and heparin-Sepharose column chromatography
-
Superdex 75 gel filtration, Mono Q column chromatography, and heparin-Sepharose column chromatography
-
three chromatographic steps
-
to homogeneity by affinity chromatography, anion-exchange chromatography and gel filtration
to homogeneity, recovery of 68% and 144fold
whole cell lysates of viable cryopreserved lymph node cells from follicular lymphomas, spelnocytes from marginal zone lymphoma, and traumatic normal spleens, and cell lines or cells are submitted to magnetic cell sorting using anti-human CD19 MACS beads
-
wild-type and recombinant enzyme
wild-type, 76fold, recombinant L-AAO expressed in and Streptomyces lividans, 186fold