1.4.3.19: glycine oxidase
This is an abbreviated version!
For detailed information about glycine oxidase, go to the full flat file.
Word Map on EC 1.4.3.19
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1.4.3.19
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d-amino
-
sarcosine
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flavoproteins
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glyphosate
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flavin
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fad
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tryptophylquinone
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flavoenzyme
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herbicide
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d-proline
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homotetrameric
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d-alanine
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pseudoalteromonas
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luteoviolacea
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protein-derived
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thios
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fad-dependent
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site-saturation
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fad-binding
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quinoprotein
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ttq
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half-reaction
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5-enolpyruvylshikimate-3-phosphate
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fad-containing
- 1.4.3.19
-
d-amino
- sarcosine
- flavoproteins
- glyphosate
- flavin
- fad
-
tryptophylquinone
-
flavoenzyme
-
herbicide
- d-proline
-
homotetrameric
- d-alanine
-
pseudoalteromonas
- luteoviolacea
-
protein-derived
-
thios
-
fad-dependent
-
site-saturation
-
fad-binding
-
quinoprotein
- ttq
-
half-reaction
- 5-enolpyruvylshikimate-3-phosphate
-
fad-containing
Reaction
Synonyms
BcGO, glycin oxidase, glycine:oxygen oxidoreductase (deaminating), GlyOX, GO, GOX, GoxA, GOXK, GoxR, LodA, PlGoxA, ThiO
ECTree
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Temperature Stability
Temperature Stability on EC 1.4.3.19 - glycine oxidase
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0 - 50
the enzyme remains stable between 0 and 50°C after 6 h incubation
34.7
melting temperature, mutant G51R/A54R, determined by following protein and flavin fluorescence
41.3
melting temperature, mutant G51R, determined by following protein and flavin fluorescence
44.8
melting temperature, mutant A54R, determined by following protein and flavin fluorescence
45
45.3
melting temperature, mutant G51S/A54R, determined by following protein and flavin fluorescence
45.7
melting temperature, mutant G51S/A54R/H244A, determined by following protein and flavin fluorescence
56.9
60
60 - 80
at 60°C, the I15V mutant shows a similar profile than the wild type enzyme but with lower residual activity after 20 min, at 65°C the mutant goes through activation (160%) during the first 15 min, the activity then falls with a time-dependent trend and reaches a half-life value similar to the wild type enzyme, at higher temperatures (70-80°C) the activity decreases rapidly in both cases, but with half-life value higher for I15V mutant than for wild type enzyme
95
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heat treatment of the protein at 95°C for 5 min results in the release of the FAD cofactor
56.9
melting temperature, wild-type, determined by following protein and flavin fluorescence
60
60 min, residual activity of wild-type is almost absent, activity of C245S mutant is 4.4%