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1.4.3.19: glycine oxidase

This is an abbreviated version!
For detailed information about glycine oxidase, go to the full flat file.

Word Map on EC 1.4.3.19

Reaction

2-iminoacetate
+
H2O
=
glyoxylate
 +
NH3

Synonyms

BcGO, glycin oxidase, glycine:oxygen oxidoreductase (deaminating), GlyOX, GO, GOX, GoxA, GOXK, GoxR, LodA, PlGoxA, ThiO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.19 glycine oxidase

Cloned

Cloned on EC 1.4.3.19 - glycine oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the glycine oxidase gene from Bacillus subtilis ATCC 6633 into the Rosetta Escherichia coli strain which contains the pRARE plasmid, inserting of the gene into the pET28a expression vector
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)CodonPlus-RIL cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli Rosetta (DE3) cells
expressed in Escherichia coli Rosetta (DE3) cells Rosetta under the strong inducible T7 promotor of the pET28a vector
-
expressed in Escherichia coli strain BL21(DE3)pLysS
-
expressed in Medicago truncatula
expression in Escherichia coli
expression in Escherichia coli BL21 (DE3)
production of a recombinant plasmid, pT7-GO by insertion of the DNA encoding for glycine oxidase into the multiple cloning site of the expression vector pT7.7. The pT7-glycine oxidase encodes a fully active fusion protein with six additional residues at the N-terminus of glycine oxidase. In BL21(DE3)pLysS Escherichia coli cells, and under optimal isopropyl thio-beta-D-galactoside induction conditions, soluble and active chimeric glycine oxidase is expressed up to 1.14 U per mg of cell. An N-terminally His-tagged glycine oxidase is also successfully expressed in Escherichia coli as a soluble protein and a fully active holoenzyme
-