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1.4.3.14: L-lysine oxidase

This is an abbreviated version!
For detailed information about L-lysine oxidase, go to the full flat file.

Word Map on EC 1.4.3.14

Reaction

L-lysine
+
O2
+
H2O
=
6-Amino-2-oxohexanoate
+
NH3
+
H2O2

Synonyms

AIP, apoptosis-inducing protein, L-LOX/MOG, L-lysine alpha-oxidase, L-lysine oxidase/monooxygenase, L-lysine-a-oxidase, L-lysine-alpha-oxidase, L-lysyl-alpha-oxidase, L6150, LyOx, lysine-oxidase, LysOx

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.14 L-lysine oxidase

Purification

Purification on EC 1.4.3.14 - L-lysine oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone precipitation, ion-exchange
-
ammonium sulfate precipitation
-
ammonium sulfate precipitation, ion-exchange
-
ammonium sulfate precipitation, ion-exchange, gel-filtration
-
DEAE-Toyopearl 650M column chromatography
-
DEAE-Toyopearl 650M column chromatography and butyl-Toyopearl 650M column chromatography
-
gel-filtration
-
L-lysine oxidase precursor prLysOX
lectin affinity column chromatography, anion-exchange HPLC, and hydroxyapatite column chromatography
octyl Sepharose column chromatography, DEAE ToyoPearl column chromatography, and gel filtration
Trichoderma cf. aureoviride
-
rapid loss of activity during isolation and purification