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1.4.3.13: protein-lysine 6-oxidase

This is an abbreviated version!
For detailed information about protein-lysine 6-oxidase, go to the full flat file.

Word Map on EC 1.4.3.13

Reaction

[protein]-L-lysine
+
O2
 +
H2O
=
[protein]-(S)-2-amino-6-oxohexanoate
 +
NH3
 +
H2O2

Synonyms

elastin synthetase, LO, LOR2, LOX, LOX-like 2, LOX-like 3, LOX-like protein, LOX-PP, LOXL, LOXL-2, LOXL1, LOXL1 protein, Loxl2, LOXL2a, Loxl3, LOXL3-sv1, LOXL3a, LOXL3b, LOXL4, LOXL5a, LOXL5b, lysyl oxidase, lysyl oxidase 1, lysyl oxidase 3, lysyl oxidase homolog 2, lysyl oxidase like 4, lysyl oxidase like-1, lysyl oxidase like-1 protein, lysyl oxidase propetide, lysyl oxidase-like, lysyl oxidase-like 1, lysyl oxidase-like 2, lysyl oxidase-like 2a, lysyl oxidase-like 2b, lysyl oxidase-like 3a, lysyl oxidase-like 3b, lysyl oxidase-like 4, lysyl oxidase-like 5a, lysyl oxidase-like 5b, lysyl oxidase-like protein, lysyl oxidase-like1 protein, lysyl oxidases, More, oxidase-like protein 1, PPLO, protein-6-oxidase, protein-L-lysine:oxygen 6-oxidoreductase, protein-lysine 6-oxidase, RAS excision protein, rDmLOXL-1, WS9-14

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.13 protein-lysine 6-oxidase

Purification

Purification on EC 1.4.3.13 - protein-lysine 6-oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
46.9fold from aorta by DEAE-cellulose ion exchange chromatography and gel filtration
-
affinity, gel-filtration, ion-exchange
-
affinity, ion-exchange
-
from aorta, to homogeneity, solubilization from connective tissue by 4-6 M urea
-
highly purified enzyme peptides
-
immobilized metal chelate column chromatography
lysyl oxidase is a highly insoluble enzyme requiring high concentrations of urea to solubilize. Development and optimization of a method to obtain lysyl oxidase in high yields directly from an Escherichia coli culture without the need for refolding of inclusion bodies using nutrient rich media, overview
-
Ni-NTA column chromatography
-
nickel affinity column chromatography and ultrafiltration
-
recombinant His-tagged mature enzyme and C-terminally deleted enzyme by nickel affinity chromatography
-
recombinant, N-terminally His6-tagged proteolytically processed forms of LOX, LOXL1, and LOXL2 from Escherichia coli inclusion bodies by nickel chelating affinity chromatography, to over 95% purity
-
truncated enzyme without the N-terminal peptide. Metal affinity chromatography under denaturing conditions followed by refolding
-