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1.4.3.11: L-glutamate oxidase

This is an abbreviated version!
For detailed information about L-glutamate oxidase, go to the full flat file.

Word Map on EC 1.4.3.11

Reaction

L-glutamate
+
O2
 +
H2O
=
2-oxoglutarate
 +
NH3
 +
H2O2

Synonyms

dehydrogenase, glutamate (acceptor), GLOD, GluOx, glutamate oxidase, glutamic acid oxidase, glutamic dehydrogenase (acceptor), GOX, L-GLOD, l-GlOx, L-glutamate oxidase, L-glutamic acid oxidase, LGOX

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.11 L-glutamate oxidase

Cloned

Cloned on EC 1.4.3.11 - L-glutamate oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli BL21(DE3) cells
expressed in Pichia pastoris GS115 cells
expression in Escherichia coli
expression in Escherichia coli strain JM109
expression in Escherichia coli. The recombinant enzyme exhibits low thermostability compared to the enzyme isolated from Streptomyces sp. X-119-6 and is an aggregated form. Proteolysis of the recombinant enzyme with metalloendopeptidase from Streptomyces griseus improves the catalytic efficiency at various pH-values and stabilizes the structure of the enzyme
-
mutant enzymes are expressed in Escherichia coli JM109 cells
overexpression in Escherichia coli JM109
-
successfully expressed by using pHBM905BDM in Pichia pastoris
-