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1.4.3.10: putrescine oxidase

This is an abbreviated version!
For detailed information about putrescine oxidase, go to the full flat file.

Word Map on EC 1.4.3.10

Reaction

putrescine
+
O2
 +
H2O
=
4-aminobutanal
 +
NH3
 +
H2O2

Synonyms

adenine dinucleotide-containing putrescine oxidase, APUO, oxidase, putrescine, PO, PuO, PUOX, PUT oxidase, PutOx, putrescine oxidase, Re-PuO

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.10 putrescine oxidase

Temperature Stability

Temperature Stability on EC 1.4.3.10 - putrescine oxidase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
pH 7.5, the enzyme retains its activity after 65 h incubation
37 - 50
at 50°C, half of the activity is lost after 2 h, while at 37°C, such a degree of inactivation is reached after 1 day
55
pH 7.5, the enzyme loses 75% of its activity after 1 h incubation at 55°C
65
-
half-life: 22.3 min, soluble enzyme. Half-life: 46.8 min, combined cross-linked enzyme aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs)
70
-
half-life: 0.89 min, soluble enzyme. Half-life: 4.7 min, combined cross-linked enzyme aggregates of a monoamine oxidase and a putrescine oxidase (combi-CLEAs)
additional information
-
the enzyme is resistant to heat denaturation as long as it is maintained in a fully reduced state