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0.047
D-Asn
mutant enzyme H56N, at pH 7.5 and 37°C
0.074
D-Leu
mutant enzyme H56N, at pH 7.5 and 37°C
172 - 5787
N-methyl-D-Asp
1.5 - 26.7
N-Methyl-D-aspartate
1.354
D-Asp
at pH 8.3 and 37°C
14.104
D-Asp
at pH 8.3 and 37°C
29.8
D-Asp
wild type enzyme, at pH 7.5 and 37°C
32.651
D-Asp
at pH 8.3 and 37°C
136
D-Asp
pH 7.0, 20°C, recombinant mutant R243K
650
D-Asp
pH 7.0, 20°C, recombinant mutant R243E
658
D-Asp
-
pH 8.3, 37°C, recombinant DDO-3
2258
D-Asp
-
pH 8.3, 37°C, recombinant DDO-2
4712
D-Asp
-
pH 8.3, 37°C, recombinant DDO-1
16500
D-Asp
pH 7.0, 20°C, recombinant mutant R243M
29800
D-Asp
pH 7.0, 20°C, recombinant wild-type enzyme
121000
D-Asp
pH 7.0, 20°C, recombinant mutant R243D
0.11
D-asparagine
pH 8.3, 25°C
0.12
D-asparagine
wild-type, pH 8.3, 25°C
14
D-Aspartate
-
wild-type, pH 7, 60°C
27.8
D-Aspartate
pH 8.3, 25°C
58.6
D-Aspartate
-
mutant F248Y, pH 7, 60°C
77.4
D-Aspartate
wild-type, pH 8.3, 25°C
0.055
D-Gln
mutant enzyme H56A, at pH 7.5 and 37°C
0.213
D-Gln
mutant enzyme H56N, at pH 7.5 and 37°C
0.024
D-Glu
wild type enzyme, at pH 7.5 and 37°C
24
D-Glu
pH 7.0, 20°C, recombinant wild-type enzyme
1117
D-Glu
-
pH 8.3, 37°C, recombinant DDO-3
1117
D-Glu
-
pH 8.3, 37°C,recombinant DDO-3
3759
D-Glu
-
pH 8.3, 37°C, recombinant DDO-2
3759
D-Glu
-
pH 8.3, 37°C,recombinant DDO-2
33440
D-Glu
-
pH 8.3, 37°C, recombinant DDO-1
0.25
D-glutamate
pH 8.3, 25°C
0.36
D-glutamate
wild-type, pH 8.3, 25°C
81
D-glutamate
-
mutant F248Y, pH 7, 60°C
100
D-glutamate
-
wild-type, pH 7, 60°C
1.3
D-glutamine
-
wild-type, pH 7, 60°C
15.5
D-glutamine
-
mutant F248Y, pH 7, 60°C
0.011
D-histidine
wild-type, pH 8.3, 25°C
0.08
D-histidine
pH 8.3, 25°C
0.125
D-Met
mutant enzyme H56N, at pH 7.5 and 37°C
1.35
D-Met
mutant enzyme H56A, at pH 7.5 and 37°C
0.119
D-Phe
mutant enzyme H56N, at pH 7.5 and 37°C
0.218
D-Phe
mutant enzyme H56A, at pH 7.5 and 37°C
0.003
D-proline
wild-type, pH 8.3, 25°C
0.03
D-proline
pH 8.3, 25°C
172
N-methyl-D-Asp
-
pH 8.3, 37°C, recombinant DDO-3
562
N-methyl-D-Asp
pH 7.0, 20°C, recombinant wild-type enzyme
654
N-methyl-D-Asp
-
pH 8.3, 37°C, recombinant DDO-2
5787
N-methyl-D-Asp
-
pH 8.3, 37°C, recombinant DDO-1
1.5
N-Methyl-D-aspartate
-
wild-type, pH 7, 60°C
10.2
N-Methyl-D-aspartate
-
mutant F248Y, pH 7, 60°C
26.7
N-Methyl-D-aspartate
pH 8.3, 25°C
26.7
N-Methyl-D-aspartate
wild-type, pH 8.3, 25°C
1.4.3.1 D-aspartate oxidase
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