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1.4.1.B2: L-erythro-3,5-diaminohexanoate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about L-erythro-3,5-diaminohexanoate dehydrogenase (NADP+), go to the full flat file.

Reaction

L-erythro-3,5-diaminohexanoate
+
H2O
 +
NADP+
=
(S)-5-amino-3-oxohexanoate
 +
NH3
 +
NADPH
 +
H+

Synonyms

3,5-DAHDH, Kdd

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.B2 L-erythro-3,5-diaminohexanoate dehydrogenase (NADP+)

Engineering

Engineering on EC 1.4.1.B2 - L-erythro-3,5-diaminohexanoate dehydrogenase (NADP+)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A179S/E310G/G323S/H328N
mutant exhibited an about 200 times enhanced activity towards substrate (R)-beta-homomethionine compared to mutant E310G and increased activities with (S)-beta-homolysine, (S)-beta-aminobutyric acid, (R)-beta-phenylalanine, (S)-beta-homophenylalanine
D177A
0.15% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D177E
7% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D177H
0.1% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D177K
almost complete loss of activity towards L-erythro-3,5-diaminohexanoate
D177N
0.15% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D177R
0.5% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D49A
loss of activity towards L-erythro-3,5-diaminohexanoate
D49E
0.1% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D49N
less than 0.1% of wild-type activity towards L-erythro-3,5-diaminohexanoate
E310G
E310G/A314Y
E310G/G323S
mutant shows improved activity towards substrate (R)-beta-homomethionine
E310S
-
the mutant exhibits activity towards (S)-beta-aminobutyric acid, beta-alanine, (R)-beta-homoserine, (R)-beta-homomethionine, (S)-beta-homoglutamine, (R)-beta-phenylalanine, and (S)-beta-homolysine
E310S/A314N
-
the mutant exhibits activity towards (S)-beta-aminobutyric acid, beta-alanine, (R)-beta-homoserine, (R)-beta-homomethionine, (S)-beta-homoglutamine, (R)-beta-phenylalanine, and (S)-beta-homolysine
H328A
71% of wild-type activity towards L-erythro-3,5-diaminohexanoate
S125A
less than 0.1% of wild-type activity towards L-erythro-3,5-diaminohexanoate
S125T
6.5% of wild-type activity towards L-erythro-3,5-diaminohexanoate
S50A
85% of wild-type activity towards L-erythro-3,5-diaminohexanoate
S50T
0.8% of wild-type activity towards L-erythro-3,5-diaminohexanoate
D177A
-
0.15% of wild-type activity towards L-erythro-3,5-diaminohexanoate
-
D177N
-
0.15% of wild-type activity towards L-erythro-3,5-diaminohexanoate
-
D49A
-
loss of activity towards L-erythro-3,5-diaminohexanoate
-
D49N
-
less than 0.1% of wild-type activity towards L-erythro-3,5-diaminohexanoate
-
S125A
-
less than 0.1% of wild-type activity towards L-erythro-3,5-diaminohexanoate
-