1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Temperature Stability

Temperature Stability on EC 1.4.1.9 - leucine dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
25
-
purified enzyme, fully stable at pH 5.0-11.0
52
-
denaturation above
53
-
unfolding temperature of mutant enzyme G78A
54
-
pH 7.0-9.5, 60 min, chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase, stable
58
-
pH 7.0-9.5, 60 min, chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase, loss of activity
76
-
unfolding temperature of mutant enzyme G79A
79
-
unfolding temperature of mutant enzyme K80R and K80Q
85
-
5 min, substantial loss of activity
94
-
10 min, 50% loss of activity, enzyme from spores
additional information
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