1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Substrates Products

Substrates Products on EC 1.4.1.9 - leucine dehydrogenase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-keto-beta-methylvalerate + NH3 + NADH
? + H2O + NAD+
show the reaction diagram
-
as active as 2-ketoisocaproate, wild-type enzyme
-
-
?
2-keto-gamma-methylthiobutanoate + NH3 + NADH
L-Met + H2O + NAD+
show the reaction diagram
-
15% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
2-ketobutyrate + NH3 + NADH
L-2-aminobutyrate + H2O + NAD+
show the reaction diagram
2-ketocaproate + NH3 + NADH
L-2-aminohexanoate + H2O + NAD+
show the reaction diagram
-
69% of the activity with 2-ketoisocaproate
-
-
?
2-ketoisocaproate + NH3 + NADH
L-Ile + H2O + NAD+
show the reaction diagram
2-ketoisovalerate + NH3 + NADH
L-Val + H2O + NAD+
show the reaction diagram
-
150% of the activity with 2-ketoisocaproate
-
-
?
2-ketovalerate + NH3 + NADH
L-norvaline + H2O + NAD+
show the reaction diagram
2-oxo-3,3-dimethylbutanoate + NH3 + NADH
L-2-amino-3,3-dimethylbutanoate + NAD+ + H2O
show the reaction diagram
-
-
-
-
?
2-oxo-4-methylselenobutyrate + NH3 + NADH
L-selenomethionine + H2O + NAD+
show the reaction diagram
-
-
-
?
2-oxobutanoate + NH3 + NADH
L-2-aminobutanoate + H2O + NAD+
show the reaction diagram
-
11% activity 2-oxobutanoate compared to 4-methyl-2-oxopentanoate
-
-
r
2-oxobutanoate + NH3 + NADH + H+
L-2-aminobutanoate + H2O + NAD+
show the reaction diagram
2-oxohexanoate + NH3 + NADH
L-norleucine + H2O + NAD+
show the reaction diagram
-
-
-
-
r
2-oxopentanoate + NH3 + NADH
L-norvaline + H2O + NAD+
show the reaction diagram
-
25% activity with 2-oxopentanoate compared to 4-methyl-2-oxopentanoate
-
-
r
2-oxophenylacetic acid + NH3 + NADH + H+
L-2-aminophenylacetic acid + H2O + NAD+
show the reaction diagram
-
-
-
-
r
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
L-tert-Leu + H2O + NAD+
show the reaction diagram
3,3-dimethyl-2-oxobutanoate + NH3 + NADH + H+
L-tert-Leu + H2O + NAD+
show the reaction diagram
-
-
-
-
r
3-methyl-2-oxobutanoate + NH3 + NADH + H+
L-valine + H2O + NAD+
show the reaction diagram
-
-
-
-
r
3-methyl-2-oxopentanoate + NH3 + NADH
L-Ile + H2O + NAD+
show the reaction diagram
-
64% activity with 3-methyl-2-oxopentanoate compared to 4-methyl-2-oxopentanoate
-
-
r
4-methyl-2-oxo-5,5,5-trifluoropentanoate + NH3 + NADH
2-amino-4-methyl-5,5,5-trifluoropentanoate + NAD+ + H2O
show the reaction diagram
-
-
-
-
?
4-methyl-2-oxopentanoate + NH3 + NADH
L-Leu + H2O + NAD+
show the reaction diagram
-
100% activity with 4-methyl-2-oxopentanoate
-
-
r
4-methyl-2-oxopentanoate + NH3 + NADH + H+
L-leucine + H2O + NAD+
show the reaction diagram
-
-
-
-
r
4-methylthio-2-oxobutyrate + NH3 + NADH
L-Met + H2O + NAD+
show the reaction diagram
-
19% activity with 4-methylthio-2-oxobutyrate compared to 4-methyl-2-oxopentanoate
-
-
r
alpha-keto beta-methylvalerate + NH3 + NADH + H+
L-isoleucine + H2O + NAD+
show the reaction diagram
yield: 95%
-
-
?
alpha-ketocaproate + NH3 + NADH + H+
L-norleucine + H2O + NAD+
show the reaction diagram
yield: 80%
-
-
?
alpha-ketoisocaproate + NH3 + NADH + H+
L-leucine + H2O + NAD+
show the reaction diagram
yield: 92.5%
-
-
?
alpha-ketoisovalerate + NH3 + NADH + H+
L-valine + H2O + NAD+
show the reaction diagram
yield: 90%
-
-
?
alpha-ketovalerate + NH3 + NADH + H+
L-norvaline + H2O + NAD+
show the reaction diagram
yield: 92%
-
-
?
L-2-aminobutanoate + H2O + NAD+
2-oxobutanoate + NH3 + NADH
show the reaction diagram
L-2-aminobutanoate + H2O + NAD+
2-oxopentanoate + NH3 + NADH
show the reaction diagram
L-2-aminobutyrate + H2O + NAD+
2-oxobutanoate + NH3 + NADH
show the reaction diagram
-
32% of the activity with L-Leu
-
-
?
L-Ala + H2O + NAD+
2-oxopropanoate + NH3 + NADH
show the reaction diagram
L-Ile + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH
show the reaction diagram
L-isoleucine + H2O + NAD+
3-methyl-2-oxopentanoate + NH3 + NADH + H+
show the reaction diagram
L-Leu + H2O + 3-acetylpyridine-deamino-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-deamino-NADH
show the reaction diagram
-
as effective as NAD+
-
-
?
L-Leu + H2O + 3-acetylpyridine-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-acetylpyridine-NADH
show the reaction diagram
-
166% of the activity with NAD+
-
-
?
L-Leu + H2O + 3-pyridinealdehyde-NAD+
4-methyl-2-oxopentanoate + NH3 + 3-pyridinealdehyde-NADH
show the reaction diagram
-
19% of the activity with NAD+
-
-
?
L-Leu + H2O + deamino-NAD+
4-methyl-2-oxopentanoate + NH3 + deamino-NADH
show the reaction diagram
-
81% of the activity with NAD+
-
-
?
L-Leu + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH
show the reaction diagram
L-Leu + H2O + thionicotinamide-NAD+
4-methyl-2-oxopentanoate + NH3 + thionicotinamide-NADH
show the reaction diagram
-
21% of the activity with NAD+
-
-
?
L-leucine + H2O + NAD+
4-methyl-2-oxopentanoate + NH3 + NADH + H+
show the reaction diagram
L-lysine + H2O + NAD+
6-amino-2-oxohexanoate + NH3 + NADH + H+
show the reaction diagram
-
69% of the activity with L-leucine
-
-
?
L-Met + H2O + NAD+
3-methylthio-2-oxobutanoate + NH3 + NADH
show the reaction diagram
L-Met + H2O + NAD+
4-methylthio-2-oxobutyrate + NH3 + NADH
show the reaction diagram
L-norleucine + H2O + NAD+
2-oxohexanoate + NH3 + NADH
show the reaction diagram
L-norleucine + H2O + NAD+
? + NH3 + NADH
show the reaction diagram
-
14% the activity with L-Leu, wild-type enzyme
-
-
?
L-norvaline + H2O + NAD+
2-ketovalerate + NH3 + NADH
show the reaction diagram
-
56% the activity with L-Leu, wild-type enzyme
-
-
?
L-norvaline + H2O + NAD+
2-oxopentanoate + NH3 + NADH
show the reaction diagram
L-Phe + H2O + NAD+
phenylpyruvate + NH3 + NADH
show the reaction diagram
-
no activity of wild-type enzyme activity with mutant enzymes A113G, A113G/V291L
-
-
?
L-S-methylcysteine + H2O + NAD+
3-methylthio-2-oxopropionate + NH3 + NADH
show the reaction diagram
-
19% of the activity with L-Leu
-
-
?
L-tert-Leu + H2O + NAD+
3,3-dimethyl-2-oxobutanoate + NH3 + NADH
show the reaction diagram
L-Val + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH
show the reaction diagram
L-valine + H2O + NAD+
3-methyl-2-oxobutanoate + NH3 + NADH + H+
show the reaction diagram
phenylpyruvate + NH3 + NADH
L-Phe + H2O + NAD+
show the reaction diagram
-
15% of the activity with 2-ketoisocaproate, wild-type enzyme
-
-
?
S-methyl-L-cysteine + H2O + NAD+
3-methylthio-2-oxopropanoate + NH3 + NADH
show the reaction diagram
-
19% the activity with L-Leu
-
-
?
trimethylpyruvate + NH3 + NADH + H+
L-tert-leucine + H2O + NAD+
show the reaction diagram
trimethylpyruvic acid + NADH + NH3 + H+
L-tert-leucine + NAD+ + H2O
show the reaction diagram
-
-
-
-
-
additional information
?
-