1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Reference

Reference on EC 1.4.1.9 - leucine dehydrogenase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Ohshima, T.; Wandrey, C.; Sugiura, M.; Soda, K.
Screening of thermostable leucine and alanine dehydrogenases in thermophilic Bacillus strains
Biotechnol. Lett.
7
871-876
1985
Bacillus sp. (in: Bacteria), Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Kataoka, K.; Takada, H.; Tanizawa, K.; Yoshimura, T.; Esaki, N.; Ohshima, T.; Soda, K.
Construction and characterization of chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase
J. Biochem.
116
931-936
1994
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Zink, M.W.; Sanwal, B.D.
L-Leucine dehydrogenase (Bacillus cereus)
Methods Enzymol.
17A
799-802
1970
Bacillus cereus
-
Manually annotated by BRENDA team
Sanwal, B.D.; Zink, M.W.
L-Leucine dehydrogenase of Bacillus cereus
Arch. Biochem. Biophys.
94
430-435
1961
Bacillus cereus
Manually annotated by BRENDA team
Zink, M.W.; Sanwal, B.D.
The distribution and substrate specificity of L-Leucine dehydrogenase
Arch. Biochem. Biophys.
99
72-77
1962
Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Bacillus pumilus, Bacillus mycoides, Bacillus niger, Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Schuette, H.; Hummel, W.; Tsai, H.; Kula, M.R.
L-Leucine dehydrogenase from Bacillus cereus
Appl. Microbiol. Biotechnol.
22
306-317
1985
Bacillus cereus
-
Manually annotated by BRENDA team
Ohshima, T.; Nagata, S.; Soda, K.
Purification and characterization of thermostable leucine dehydrogenase from Bacillus stearothermophilus
Arch. Microbiol.
141
407-411
1985
Geobacillus stearothermophilus
-
Manually annotated by BRENDA team
Livesey, G.; Lund, P.
Isolation and characterization of leucine dehydrogenase from Bacillus subtilis
Methods Enzymol.
166
282-288
1988
Bacillus subtilis
Manually annotated by BRENDA team
Kaerst, U.; Schuette, H.; Baydoun, H.; Tsai, H.
Purification and characterization of leucine dehydrogenase from the thermophile Bacillus caldolyticus
J. Gen. Microbiol.
135
1305-1313
1989
[Bacillus] caldolyticus
-
Manually annotated by BRENDA team
Ohshima, T.; Misono, H.; Soda, K.
Properties of crystalline leucine dehydrogenase from Bacillus sphaericus
J. Biol. Chem.
253
5719-5725
1978
Brevibacillus brevis, Bacillus cereus, Bacillus megaterium, Bacillus subtilis, Bacillus pumilus, Lysinibacillus sphaericus
Manually annotated by BRENDA team
Obermeier, N.; Poralla, K.
Some physiological functions of the L-leucine dehydrogenase in Bacillus subtilis
Arch. Microbiol.
109
59-63
1976
Bacillus subtilis
Manually annotated by BRENDA team
Ohshima, T.; Yamamoto, T.; Misono, H.; Soda, K.
Leucine dehydrogenase of bacillus sphaericus: sulfhydryl groups and catalytic sites
Agric. Biol. Chem.
42
1739-1743
1978
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Hummel, W.; Schuette, H.; Kula, M.R.
Leucine dehydrogenase from Bacillus sphaericus. Optimized production conditions and efficient method for its large-scale purification
Eur. J. Appl. Microbiol. Biotechnol.
12
22-27
1981
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Nagata, S.; Tanizawa, K.; Esaki, N.; Sakamoto, Y.; Ohshima, T.; Tanaka, H.; Soda, K.
Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases
Biochemistry
27
9056-9062
1988
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Schustolla, D.; Hustedt, H.
Gene cloning and sequence determination of leucine dehydrogenase from Bacillus stearothermophilus and structural comparison with other NAD(P)+-dependent dehydrogenases
DECHEMA Biotechnol. Conf.
3
1097-1101
1989
Bacillus cereus
-
Manually annotated by BRENDA team
Obermeier, N.; Poralla, K.
Experiments on the role of leucine dehydrogenase in initiation of Bacillus subtilis spore germination
FEMS Microbiol. Lett.
5
81-83
1979
Bacillus subtilis, Bacillus subtilis 168M
-
Manually annotated by BRENDA team
Oki, T.; Shirai, M.
Antitumor activities of bacterial leucine dehydrogenase and glutaminase A
FEBS Lett.
33
286-288
1973
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Ohshima, T.; Soda, K.
Modification of leucine dehydrogenase by pyridoxal 5'-phosphate
Agric. Biol. Chem.
48
349-354
1984
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Oka, M.; Yang, Y.S.; Nagata, S.; Esaki, N.; Tanaka, H.; Soda, K.
Overproduction of thermostable leucine dehydrogenase of Bacillus stearothermophilus and its one-step purification from recombinant cells of Escherichia coli
Biotechnol. Appl. Biochem.
11
307-311
1989
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Soda, K.; Misono, H.; Mori, K.; Sakato, H.
Crystalline L-leucine dehydrogenase
Biochem. Biophys. Res. Commun.
44
931-935
1971
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Esaki, N.; Shimoi, H.; Yang, Y.S.; Tanaka, H.; Soda, K.
Enantioselective synthesis of L-selenomethionine with leucine dehydrogenase
Biotechnol. Appl. Biochem.
11
312-317
1989
Geobacillus stearothermophilus
-
Manually annotated by BRENDA team
Kiba, N.; Hori, S.; Furusawa, M.
Flow-injection determination of branched-chain L-amino acids with immobilized leucine dehydrogenase
Anal. Chim. Acta
218
161-166
1989
Bacillus cereus, Bacillus sp. (in: Bacteria)
-
Manually annotated by BRENDA team
Warth, A.D.
Heat stability of Bacillus cereus enzymes within spores and in extracts
J. Bacteriol.
143
27-34
1980
Bacillus cereus
Manually annotated by BRENDA team
Shimoi, H.; Nagata, S.; Esaki, N.; Tanaka, H.; Soda, K.
Leucine dehydrogenase of a thermophilic anaerobe, Clostridium thermoaceticum: gene cloning, purification and characterization
Agric. Biol. Chem.
51
3375-3381
1987
Moorella thermoacetica
-
Manually annotated by BRENDA team
Nagata, S.; Misono, H.; Nagasaki, S.; Esaki, N.; Tanaka, H.; Soda, K.
Gene cloning, purification, and characterization of the highly thermostable leucine dehydrogenase of bacillus sp.
J. Ferment. Bioeng.
69
199-203
1990
Bacillus sp. (in: Bacteria)
-
Manually annotated by BRENDA team
Nagata, S.; Bakthavatsalam, S.; Galkin, A.G.; Asada, H.; Sakai, S.; Esaki, N.; Soda, K.; Ohshima, T.; Nagasaki, S.; Misono, H.
Gene cloning, purification, and characterization of thermostable and halophilic leucine dehydrogenase from a halophilic thermophile, Bacillus licheniformis TSN9
Appl. Microbiol. Biotechnol.
44
432-438
1995
Bacillus licheniformis
Manually annotated by BRENDA team
Ansorge, M.B.; Kula, M.R.
Production of recombinant L-leucine dehydrogenase from Bacillus cereus in pilot scale using the runaway replication system E. coli[pIET98]
Biotechnol. Bioeng.
68
557-562
2000
Bacillus cereus
Manually annotated by BRENDA team
Ohshima, T.; Nishida, N.; Bakthavatsalam, S.; Kataoka, K.; Takada, H.; Yoshimura, T.; Esaki, N.; Soda, K.
The purification, characterization, cloning and sequencing of the gene for a halostable and thermostable leucine dehydrogenase from Thermoactinomyces intermedius
Eur. J. Biochem.
222
305-312
1994
Thermoactinomyces intermedius
Manually annotated by BRENDA team
Galkin, A.; Kulakova, L.; Ohshima, T.; Esaki, N.; Soda, K.
Construction of a new leucine dehydrogenase with preferred specificity for NADP+ by site-directed mutagenesis of the strictly NAD+-specific enzyme
Protein Eng.
10
687-690
1997
Thermoactinomyces intermedius
Manually annotated by BRENDA team
Sekimoto, T.; Fukui, T.; Tanizawa, K.
Role of the conserved glycyl residues located at the active site of leucine dehydrogenase from Bacillus stearothermophilus
J. Biochem.
116
176-182
1994
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Baker, P.J.; Turnbull, A.P.; Sedelnikova, S.E.; Stillman, T.J.; Rice, D.W.
A role for quaternary structure in the substrate specificity of leucine dehydrogenase
Structure
3
693-705
1995
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Kiba, N.; Oyama, Y.; Kato, A.; Furusawa, M.
Postcolumn co-immobilized leucine dehydrogenase-NADH oxidase reactor for the determination of branched-chain amino acids by high-performance liquid chromatography with chemiluminescence detection
J. Chromatogr. A
724
354-357
1996
Geobacillus stearothermophilus
-
Manually annotated by BRENDA team
Sekimoto, T.; Fukui, T.; Tanizawa, K.
Involvement of conserved lysine 68 of Bacillus stearothermophilus leucine dehydrogenase in substrate binding
J. Biol. Chem.
269
7262-7266
1994
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Sekimoto, T.; Matsuyama, T.; Fukui, T.; Tanizawa, K.
Evidence for lysine 80 as general base catalyst of leucine dehydrogenase
J. Biol. Chem.
268
27039-27045
1993
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Muranova, T.A.; Ruzheinikov, S.N.; Sedelnikova, S.E.; Baker, P.J.; Pasquo, A.; Galkin, A.; Esaki, N.; Ohshima, T.; Soda, K.; Rice, D.W.
Crystallization and preliminary X-ray analysis of substrate complexes of leucine dehydrogenase from Thermoactinomyces intermedius
Acta Crystallogr. Sect. D
58
1059-1062
2002
Lysinibacillus sphaericus, Thermoactinomyces intermedius
Manually annotated by BRENDA team
Turnbull, A.P.; Ashford, S.R.; Baker, P.J.; Rice, D.W.; Rodgers, F.H.; Stillman, T.J.; Hanson, R.L.
Crystallization and quaternary structure analysis of the NAD+-dependent leucine dehydrogenase from Bacillus sphaericus
J. Mol. Biol.
236
663-665
1994
Lysinibacillus sphaericus
Manually annotated by BRENDA team
Oikawa, T.; Kataoka, K.; Jin, Y.; Suzuki, S.; Soda, K.
Fragmentary form of thermostable leucine dehydrogenase of Bacillus stearothermophilus: its construction and reconstitution of active fragmentary enzyme
Biochem. Biophys. Res. Commun.
280
1177-1182
2001
Geobacillus stearothermophilus
Manually annotated by BRENDA team
Gu, K.F.; Chang, M.S.
Production of essential L-branched-chain amino acids in bioreactors containing artificial cells immobilized multienzyme system and dextran-NAD+
Biotechnol. Bioeng.
36
263-269
1990
Bacillus sp. (in: Bacteria)
Manually annotated by BRENDA team
Gu, K.F.; Chang, M.S.
Conversion of ammonia or urea into essential amino acids, L-leucine, L-valine, and L-isoleucine, using artificial cells containing as immobilized multienzyme system and dextran-NAD+
Biotechnol. Appl. Biochem.
12
227-236
1990
Bacillus sp. (in: Bacteria)
Manually annotated by BRENDA team
Livesey, G.; Lund, P.
Determination of branched-chain amino and keto acids with leucine dehydrogenase
Methods Enzymol.
166
3-10
1988
Bacillus subtilis, Lysinibacillus sphaericus
Manually annotated by BRENDA team
Morishita, Y.; Nakane, K.; Fukatsu, T.; Nakashima, N.; Tsuji, K.; Soya, Y.; Yoneda, K.; Asano, S.; Kawamura, Y.
Kinetic assay of serum and urine for urea with use of urease and leucine dehydrogenase
Clin. Chem.
43
1932-11936
1997
Bacillus subtilis
Manually annotated by BRENDA team
Kiba, N.; Muto, M.; Furusawa, M.
High-performance liquid chromatographic determination of branched-chain alpha-keto acids in serum using immobilized leucine dehydrogenase as post-column reactor
J. Chromatogr.
497
236-242
1989
Bacillus subtilis
Manually annotated by BRENDA team
Katoh, R.; Ngata, S.; Ozawa, A.; Ohshima, T.; Kamekura, M.; Misono, H.
Purification and characterization of leucine dehydrogenase from an alkalophilic halophile, Natronobacterium magadii MS-3
J. Mol. Catal. B
23
231-238
2003
Natrialba magadii, Natrialba magadii MS-3
-
Manually annotated by BRENDA team
Katoh, R.; Nagata, S.; Misono, H.
Cloning and sequencing of the leucine dehydrogenase gene from Bacillus sphaericus IFO 3525 and importance of the C-terminal region for the enzyme activity
J. Mol. Catal. B
23
239-247
2003
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Kataoka, K.; Tanizawa, K.
Alteration of substrate specificity of leucine dehydrogenase by site-directed mutagenesis
J. Mol. Catal. B
23
299-309
2003
Geobacillus stearothermophilus
-
Manually annotated by BRENDA team
Bradshaw, C.W.; Wong, C.H.; Hummel, W.; Kula, M.R.
Enzyme-catalyzed asymetric synthesis of (S)-2-amino-4-phenylbutanoic acid and (R)-2-hydroxy-4-phenylbutanoic acid
Bioorg. Chem.
19
29-39
1991
Lysinibacillus sphaericus
-
Manually annotated by BRENDA team
Chiriac, M.; Lupan, I.; Bucurenci, N.; Popescu, O.; Palibroda, N.
Stereoselective synthesis of L-[15N] amino acids with glucose dehydrogenase and galactose mutarotase as NADH regenerating system
J. Labelled Compd. Radiopharm.
51
171-174
2008
Geobacillus stearothermophilus (P13154)
-
Manually annotated by BRENDA team
Li, H.; Zhu, D.; Hyatt, B.A.; Malik, F.M.; Biehl, E.R.; Hua, L.
Cloning, protein sequence clarification, and substrate specificity of a leucine dehydrogenase from Bacillus sphaericus ATCC4525
Appl. Biochem. Biotechnol.
158
343-351
2008
Lysinibacillus sphaericus, Lysinibacillus sphaericus ATCC 4525
Manually annotated by BRENDA team
Zhao, Y.; Wakamatsu, T.; Doi, K.; Sakuraba, H.; Ohshima, T.
A psychrophilic leucine dehydrogenase from Sporosarcina psychrophila: purification, characterization, gene sequencing and crystal structure analysis
J. Mol. Catal. B
83
65-72
2012
Sporosarcina psychrophila, Sporosarcina psychrophila DSM 3
-
Manually annotated by BRENDA team
Parker, W.; Hanson, R.; Goldberg, S.; Tully, T.; Goswami, A.
Preparation of (S)-1-cyclopropyl-2-methoxyethanamine by a chemoenzymatic route using leucine dehydrogenase
Org. Process Res. Dev.
16
464-469
2012
Thermoactinomyces intermedius
-
Manually annotated by BRENDA team
Lu, J.; Zhang, Y.; Sun, D.; Jiang, W.; Wang, S.; Fang, B.
The development of leucine dehydrogenase and formate dehydrogenase bifunctional enzyme cascade improves the biosynthsis of L-tert-teucine
Appl. Biochem. Biotechnol.
180
1180-1195
2016
Bacillus cereus
Manually annotated by BRENDA team
Xu, J.M.; Cheng, F.; Fu, F.T.; Hu, H.F.; Zheng, Y.G.
Semi-rational engineering of leucine dehydrogenase for L-2-aminobutyric acid production
Appl. Biochem. Biotechnol.
182
898-909
2016
Bacillus cereus
Manually annotated by BRENDA team
Zhu, L.; Wu, Z.; Jin, J.M.; Tang, S.Y.
Directed evolution of leucine dehydrogenase for improved efficiency of L-tert-leucine synthesis
Appl. Microbiol. Biotechnol.
100
5805-5813
2016
Lysinibacillus sphaericus (Q76GS2), Lysinibacillus sphaericus
Manually annotated by BRENDA team
Liu, W.; Ma, H.; Luo, J.; Shen, W.; Xu, X.; Li, S.; Hu, Y.; Huang, H.
Efficient synthesis of L-tert-leucine through reductive amination using leucine dehydrogenase and formate dehydrogenase coexpressed in recombinant E. coli
Biochem. Eng. J.
91
204-209
2014
Lysinibacillus sphaericus (Q76GS2), Lysinibacillus sphaericus CGMCC 1.1677 (Q76GS2)
-
Manually annotated by BRENDA team
Tao, R.; Jiang, Y.; Zhu, F.; Yang, S.
A one-pot system for production of L-2-aminobutyric acid from L-threonine by L-threonine deaminase and a NADH-regeneration system based on L-leucine dehydrogenase and formate dehydrogenase
Biotechnol. Lett.
36
835-841
2014
Bacillus cereus (P0A392), Bacillus cereus, Bacillus cereus DSM 31 (P0A392)
Manually annotated by BRENDA team
Zhu, W.; Li, Y.; Jia, H.; Wei, P.; Zhou, H.; Jiang, M.
Expression, purification and characterization of a thermostable leucine dehydrogenase from the halophilic thermophile Laceyella sacchari
Biotechnol. Lett.
38
855-861
2016
Laceyella sacchari
Manually annotated by BRENDA team
Jiang, W.; Sun, D.; Lu, J.; Wang, Y.; Wang, S.; Zhang, Y.; Fang, B.
A cold-adapted leucine dehydrogenase from marine bacterium Alcanivorax dieselolei Characterization and l-tert-leucine production
Eng. Life Sci.
16
283-289
2016
Alcanivorax dieselolei
-
Manually annotated by BRENDA team
Zhang, Y.; Wang, Y.; Wang, S.; Fang, B.
Engineering bi-functional enzyme complex of formate dehydrogenase and leucine dehydrogenase by peptide linker mediated fusion for accelerating cofactor regeneration
Eng. Life Sci.
17
989-996
2017
Bacillus cereus (Q731C9), Bacillus cereus ATCC 10987 (Q731C9)
-
Manually annotated by BRENDA team
Mahdizadehdehosta, R.; Kianmehr, A.; Khalili, A.
Isolation and characterization of leucine dehydrogenase from a thermophilic Citrobacter freundii JK-91 strain isolated from Jask Port
Iran. J. Microbiol.
5
278-284
2013
Citrobacter freundii, Citrobacter freundii JK-91
Manually annotated by BRENDA team
Li, J.; Pan, J.; Zhang, J.; Xu, J.
Stereoselective synthesis of L-tert-leucine by a newly cloned leucine dehydrogenase from Exiguobacterium sibiricum
J. Mol. Catal. B
105
11-17
2014
Exiguobacterium sibiricum
-
Manually annotated by BRENDA team