1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

Purification

Purification on EC 1.4.1.9 - leucine dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity extraction
-
chimeric enzyme consisting of an amino-terminal domain of phenylalanine dehydrogenase and a carboxy-terminal domain of leucine dehydrogenase
-
mutant enzymes K68A and K68R
-
native enzyme 48fold by ammonium sulfate fractionation, anion exchange and hydrophobic interaction chromatography , and gel filtration to over 95% purity
-
Ni-NTA agarose gel chromatography
-
one-step purification of recombinant enzyme
-
recombinant enzyme