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0.194 - 0.902
2-oxo-3-methylbutanoate
0.0025 - 0.238
2-oxobutanoate
0.232 - 0.337
2-oxopentanoate
0.09 - 13
3,3-dimethyl-2-oxobutanoate
0.309 - 1.44
4-methyl-2-oxopentanoate
22.1
DL-methionine
pH 9, 30°C
16.8
L-arginine
pH 9, 30°C
32.5
L-proline
pH 9, 30°C
6.7
L-tert-leucine
Roseibium aggregatum
-
wild-type, pH 8, 30°C
0.019 - 0.191
oxo(phenyl)acetate
-
0.004 - 7165
trimethylpyruvate
0.194
2-oxo-3-methylbutanoate
wild-type, pH 9.5, 35°C
0.276
2-oxo-3-methylbutanoate
mutant T45M, pH 9.5, 35°C
0.413
2-oxo-3-methylbutanoate
mutant T45M/E116V, pH 9.5, 35°C
0.902
2-oxo-3-methylbutanoate
mutant E116V, pH 9.5, 35°C
0.0025
2-oxobutanoate
-
wild-type, pH 7.5, 37°C
0.0217
2-oxobutanoate
-
mutant M374G, pH 7.5, 37°C
0.022
2-oxobutanoate
-
mutant M347G, pH 7.5, 37°C
0.0492
2-oxobutanoate
-
mutant M347G/Q358N, pH 7.5, 37°C
0.05
2-oxobutanoate
-
mutant M347G/Q358N, pH 7.5, 37°C
0.079
2-oxobutanoate
wild-type, pH 9.5, 35°C
0.102
2-oxobutanoate
mutant T45M, pH 9.5, 35°C
0.107
2-oxobutanoate
mutant T45M/E116V, pH 9.5, 35°C
0.11
2-oxobutanoate
-
mutant Q358N, pH 7.5, 37°C
0.1112
2-oxobutanoate
-
mutant Q358N, pH 7.5, 37°C
0.238
2-oxobutanoate
mutant E116V, pH 9.5, 35°C
0.232
2-oxopentanoate
wild-type, pH 9.5, 35°C
0.295
2-oxopentanoate
mutant T45M, pH 9.5, 35°C
0.315
2-oxopentanoate
mutant T45M/E116V, pH 9.5, 35°C
0.337
2-oxopentanoate
mutant E116V, pH 9.5, 35°C
0.09
3,3-dimethyl-2-oxobutanoate
-
His-tagged enzyme, pH 9.0, 25°C
0.1
3,3-dimethyl-2-oxobutanoate
-
pH 7.0, temperature not specified in the publication
0.26
3,3-dimethyl-2-oxobutanoate
-
enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C
2.6
3,3-dimethyl-2-oxobutanoate
wild-type, pH 10.0, 30°C
13
3,3-dimethyl-2-oxobutanoate
mutant A43V/D124E , pH 10.0, 30°C
0.309
4-methyl-2-oxopentanoate
mutant T45M, pH 9.5, 35°C
0.332
4-methyl-2-oxopentanoate
wild-type, pH 9.5, 35°C
0.433
4-methyl-2-oxopentanoate
mutant T45M/E116V, pH 9.5, 35°C
1.44
4-methyl-2-oxopentanoate
mutant E116V, pH 9.5, 35°C
4.9
L-leucine
pH 8.5, 30°C
23.3
L-leucine
-
pH 9.5, 30°C
91.3
L-leucine
pH 9, 30°C
5.8
NAD+
pH 8.5, 30°C
434.6
NAD+
Roseibium aggregatum
-
wild-type, pH 8, 30°C
2.46
NADH
mutant T45M, pH 9.5, 35°C
3.18
NADH
mutant T45M/E116V, pH 9.5, 35°C
3.18
NADH
wild-type, pH 9.5, 35°C
11
NADH
-
pH 7.0, temperature not specified in the publication
18.7
NADH
mutant E116V, pH 9.5, 35°C
21.8
NADH
-
His-tagged enzyme, pH 9.0, 25°C
43
NADH
Roseibium aggregatum
-
wild-type, pH 8, 30°C
51.3
NADH
-
enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C
118.5
NADH
wild-type, pH 8.5, 30°C
188.6
NADH
mutant D126E, pH 8.5, 30°C
241.9
NADH
mutant E116V, pH 8.5, 30°C
276.8
NADH
mutant E24A/D126E, pH 8.5, 30°C
350.9
NADH
mutant E24V/E116V, pH 8.5, 30°C
480
NADH
wild-type, pH 10.0, 30°C
808
NADH
Roseibium aggregatum
-
wild-type, pH 8, 30°C
2800
NADH
mutant A43V/D124E , pH 10.0, 30°C
7936
NADH
Roseibium aggregatum
-
mutant H191N, pH 8, 30°C
12348
NADH
Roseibium aggregatum
-
mutant P130V, pH 8, 30°C
15013
NADH
Roseibium aggregatum
-
mutant D153N, pH 8, 30°C
40465
NADH
Roseibium aggregatum
-
mutant D153N/H191N, pH 8, 30°C
0.019
oxo(phenyl)acetate
wild-type, pH 9.5, 35°C
-
0.03
oxo(phenyl)acetate
mutant T45M, pH 9.5, 35°C
-
0.134
oxo(phenyl)acetate
mutant E116V, pH 9.5, 35°C
-
0.191
oxo(phenyl)acetate
mutant T45M/E116V, pH 9.5, 35°C
-
0.004
trimethylpyruvate
wild-type, pH 9.5, 35°C
0.005
trimethylpyruvate
mutant T45M, pH 9.5, 35°C
0.014
trimethylpyruvate
mutant T45M/E116V, pH 9.5, 35°C
0.019
trimethylpyruvate
mutant E116V, pH 9.5, 35°C
1.55
trimethylpyruvate
wild-type, pH 8.5, 30°C
3.34
trimethylpyruvate
mutant D126E, pH 8.5, 30°C
5.31
trimethylpyruvate
mutant E24A/D126E, pH 8.5, 30°C
5.63
trimethylpyruvate
mutant E116V, pH 8.5, 30°C
7.6
trimethylpyruvate
pH 8.5, 30°C
8.36
trimethylpyruvate
mutant E24V/E116V, pH 8.5, 30°C
12.7
trimethylpyruvate
-
pH 9.5, 30°C
2058
trimethylpyruvate
Roseibium aggregatum
-
mutant D153N, pH 8, 30°C
3618
trimethylpyruvate
Roseibium aggregatum
-
mutant P130V, pH 8, 30°C
3620
trimethylpyruvate
Roseibium aggregatum
-
wild-type, pH 8, 30°C
4794
trimethylpyruvate
Roseibium aggregatum
-
mutant H191N, pH 8, 30°C
7165
trimethylpyruvate
Roseibium aggregatum
-
mutant D153N/H191N, pH 8, 30°C
1.4.1.9 leucine dehydrogenase
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