1.4.1.8: valine dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about valine dehydrogenase (NADP+), go to the full flat file.

Reaction

Synonyms

dehydrogenase, valine (nicotinamide adenine dinucleotide phosphate), ValDH, valine dehydrogenase (nicotinamide adenine dinucleotide phosphate), VDH

ECTree

     1 Oxidoreductases

         1.4 Acting on the CH-NH₂ group of donors

             1.4.1 With NAD⁺ or NADP⁺ as acceptor

                1.4.1.8 valine dehydrogenase (NADP+)

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Results	in table
7	Activating Compound
15	AA Sequence
1	CAS Registry Number
7	Cloned(Commentary)
83	Cofactor
1	General Stability
33	Inhibitors
1	Ki Value [mM]
90	KM Value [mM]
22	Localization
14	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
51	Organism
26	pH Optimum
2	pH Range
3	pH Stability
7	Protein Variants
12	Purification (Commentary)
2	Reaction
5	Reaction Type
35	Reference
25	Source Tissue
41	Specific Activity [micromol/min/mg]
8	Storage Stability
282	Substrates and Products (Substrate)
11	Subunits
5	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
1	Temperature Stability [°C]
20	Turnover Number [1/s]

Engineering

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Engineering on EC 1.4.1.8 - valine dehydrogenase (NADP+)

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PROTEIN VARIANTS

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

A124G

Streptomyces albus

-

lower activity toward aliphatic amino acid substrates, e.g. catalytic rate constant for oxidative deamination of L-valine is only 12% of the wild type, but higher activities toward L-phenylalanine, L-tyrosine, and L-methionine

391700

C251S

Streptomyces albus

-

kcat for deamination is 13% of the wild-type value

675330

C30S

Streptomyces albus

-

kcat for deamination is 13% of the wild-type value

675330

K79A

Streptomyces albus

-

lower affinity for L-valine and 2-oxoisovalerate and lower catalytic rate constants compared to the wild type enzyme

391701

K91A

Streptomyces albus

-

lower affinity for L-valine and 2-oxoisovalerate and lower catalytic rate constants compared to the wild type enzyme

391701

V305L

Streptomyces albus

-

kcat for deamination is 0.1% of the wild-type value

675330

additional information

Streptomyces albus

-

active site lysine residue modified with pyridoxal 5’-phosphate

391701