preparation of glutamate sensors using NAD- and NADP-dependent glutamate dehydrogenase (GDH). Glutamate is determined from the anodic oxidation current of NADH and NADPH generated by the enzymatic reaction of GDH. Enzymes are deposited separately on carbon nanotube and reduced graphene oxide-modified glassy carbon electrodes
in the parasite protein, several salt-bridges mediate contacts between the subunits, possesses a unique N-terminal extension that does not occur in any other GDH sequence so far studied, design of peptidomimetics capable of disrupting the oligomeric organisation of the parasite enzyme
in presence of polyethyleneimine , the enzyme almost maintains the full initial activity after 2 h under conditions where the untreated enzyme retains only 20% of the initial activity, and the effect of the enzyme concentration on enzyme stability almost disappears. This stabilization is maintained in the pH range 59, but it is lost at high ionic strength. This polyethyleneimine -GDH composite is much more stable than the unmodified enzyme in stirred systems
expression in Oryza sativa. At the seedling stage, the leaf area and shoot and root dry weights of the high gdhA-expressors are higher than those of control plants under both high (high N) and low nitrogen (low N) conditions. The net photosynthesis rate at the heading stage is higher in transgenic than in control leaves. Under both high and low N conditions, the nitrogen contents in the shoots and roots, at seedling and grain-harvest stages, are significantly higher in high gdhA-expressors than in control plants. At the harvest stage, the high gdhA-expressors exhibit greater panicle and spikelet numbers per plant compared with control plants, resulting in higher grain weight. In addition, gdhA expression in forage rice significantly enhances their tolerance to salt stress compared to control plants
is responsible for the capacity of Lactococcus lactis strains to catabolize amino acids to aroma compounds, pGdh442 can be naturally transmitted to other Lactococcus lactis strains by using cadmium or zinc resistance as a selectable marker, new prospects to intensify and diversify the production of aroma compounds in cheese
is responsible for the capacity of Lactococcus lactis strains to catabolize amino acids to aroma compounds, pGdh442 can be naturally transmitted to other Lactococcus lactis strains by using cadmium or zinc resistance as a selectable marker, new prospects to intensify and diversify the production of aroma compounds in cheese
in order for the wild-type nitrogen assimilation control protein to exert strong repression of gdhA, it must form a tetramer that bridges the two sites at gdhA, similar to other DNA looping models, the negative control mutants of nitrogen assimilation control protein fail to tetramerize and cannot form this loop, thus fail to exert the strong repression at gdhA
lack of a structure called antenna, NAD(P)-binding motif GAGNVA, and a second putative coenzyme-binding motif GVLTGKG together with the four residues Lys, Ser, Arg and Thr involved in the binding of the reduced form of NADP, key role of GDH4 in ammonium assimilation
lack of a structure called antenna, NAD(P)-binding motif GAGNVA, and a second putative coenzyme-binding motif GVLTGKG together with the four residues Lys, Ser, Arg and Thr involved in the binding of the reduced form of NADP, key role of GDH4 in ammonium assimilation
in order for the wild-type nitrogen assimilation control protein to exert strong repression of gdhA, it must form a tetramer that bridges the two sites at gdhA, similar to other DNA looping models, the negative control mutants of nitrogen assimilation control protein fail to tetramerize and cannot form this loop, thus fail to exert the strong repression at gdhA