1.4.1.28: secondary-alkyl amine dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about secondary-alkyl amine dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
a secondary-alkyl amine +
H2O +
NAD(P)+=
a ketone +
NH3 +
NAD(P)H +
H+
Synonyms
AMDH, AmDH4, amine dehydrogenase, D187_009359, DapB_1, NAD+-dependent amine dehydrogenase, Pmob_1166
ECTree
KM Value
KM Value on EC 1.4.1.28 - secondary-alkyl amine dehydrogenase [NAD(P)+]
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0.326 - 0.894
(2R)-2-amino-4-oxopentanoate
1.48 - 3.64
1-hydroxybutan-2-one
2.32
2-methylcyclohexanone
pH 9.5, 23°C
0.326
(2R)-2-amino-4-oxopentanoate
wild-type, pH 9.5, 23°C
0.894
(2R)-2-amino-4-oxopentanoate
mutant E102A, pH 9.5, 23°C
1.48
1-hydroxybutan-2-one
pH 8.5, 50°C
2.32
1-hydroxybutan-2-one
pH 9.0, 55°C
2.42
1-hydroxybutan-2-one
pH 8.5, 50°C
3.44
1-hydroxybutan-2-one
pH 8.5, 55°C
3.64
1-hydroxybutan-2-one
pH 8.5, 45°C
0.55
cyclohexanone
pH 9.5, 23°C
2.47
cyclohexanone
pH 9.5, 23°C
2.5
cyclohexanone
pH 9.5, 23°C
2.2
dihydroxyacetone
-
pH 9, 25°C
2.2
dihydroxyacetone
-
pH 7, 25°C
0.54
Isobutanal
pH 9.5, 23°C
0.86
Isobutanal
pH 9.5, 23°C
0.84
NAD+
-
pH 8, 25°C
0.022
NADH
-
pH 9, 25°C
0.025
NADH
wild-type, pH 9.5, 23°C
0.01
NADPH
pH 9.5, 23°C
26.5
NH3
-
pH 9, 25°C
319.7
NH3
wild-type, pH 9.5, 23°C
4
serinol
-
pH 8, 25°C
1.4.1.28 secondary-alkyl amine dehydrogenase [NAD(P)+]
results (
results (
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