1.4.1.28: secondary-alkyl amine dehydrogenase [NAD(P)+]
This is an abbreviated version!
For detailed information about secondary-alkyl amine dehydrogenase [NAD(P)+], go to the full flat file.
Reaction
a secondary-alkyl amine +
H2O +
NAD(P)+=
a ketone +
NH3 +
NAD(P)H +
H+
Synonyms
AMDH, AmDH4, amine dehydrogenase, D187_009359, DapB_1, NAD+-dependent amine dehydrogenase, Pmob_1166
ECTree
KCat KM Value
KCat KM Value on EC 1.4.1.28 - secondary-alkyl amine dehydrogenase [NAD(P)+]
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2.81 - 130
(2R)-2-amino-4-oxopentanoate
0.09 - 0.71
1-hydroxybutan-2-one
0.26 - 0.261
2-methylcyclohexanone
0.054 - 54.4
cyclohexanone
2.81
(2R)-2-amino-4-oxopentanoate
mutant E102A, pH 9.5, 23°C
130
(2R)-2-amino-4-oxopentanoate
wild-type, pH 9.5, 23°C
0.09
1-hydroxybutan-2-one
pH 8.5, 45°C
0.31
1-hydroxybutan-2-one
pH 8.5, 50°C
0.44
1-hydroxybutan-2-one
pH 8.5, 55°C
0.52
1-hydroxybutan-2-one
pH 8.5, 50°C
0.71
1-hydroxybutan-2-one
pH 9.0, 55°C
0.26
2-methylcyclohexanone
pH 9.5, 23°C
0.261
2-methylcyclohexanone
pH 9.5, 23°C
0.054
cyclohexanone
pH 9.5, 23°C
1.72
cyclohexanone
pH 9.5, 23°C
1.723
cyclohexanone
pH 9.5, 23°C
54.4
cyclohexanone
pH 9.5, 23°C
0.209
Isobutanal
pH 9.5, 23°C
0.62
Isobutanal
pH 9.5, 23°C
1.705
NADH
pH 9.5, 23°C
1440
NADH
wild-type, pH 9.5, 23°C
4.537
NADPH
pH 9.5, 23°C
0.0004
NH3
pH 9.5, 23°C
0.118
NH3
wild-type, pH 9.5, 23°C
1.4.1.28 secondary-alkyl amine dehydrogenase [NAD(P)+]
results (
results (
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