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1.4.1.13: glutamate synthase (NADPH)

This is an abbreviated version!
For detailed information about glutamate synthase (NADPH), go to the full flat file.

Word Map on EC 1.4.1.13

Reaction

2 L-glutamate +

NADP+
=
L-glutamine
 +
2-oxoglutarate
 +
NADPH
 +
H+

Synonyms

EC 2.6.1.53, EhNO1, EhNO2, gltA, gltB, GltB1, GltB2, gltD, GltS, glutamate synthetase (NADP), glutamine amide-2-oxoglutarate aminotransferase (oxidoreductase, NADP), glutamine-ketoglutaric aminotransferase, GOGAT, L-glutamate synthase, L-glutamate synthetase, L-glutamine:2-oxoglutarate aminotransferase, NADPH oxidizing, NADPH-dependent glutamate synthase, NADPH-GltS, NADPH-glutamate synthase, NADPH-GOGAT, NADPH-linked glutamate synthase, pGLTY1, pGLTY2, pGLTZ, PH0876, PH1873, synthase, glutamate (reduced nicotinamide adenine dinucleotide phosphate)

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.13 glutamate synthase (NADPH)

Purification

Purification on EC 1.4.1.13 - glutamate synthase (NADPH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
of the alpha subunit, using column chromatography on Q-Sepharose and Ultrogel AcA34
-
of the G298A-beta subunit, using column chromatography on Reactive Red or Amicon Red resins and gel filtration on Ultrogel AcA 34
-
of the recombinant enzyme beta subunit, using ammonium sulfate fractionation, affinity chromatography on Reactive Red, ultrafiltration and column chromatography on Ultrogel AcA 54
-
of the recombinant enzyme from overproducing Escherichia coli cells, using ion exchange chromatography on Q-Sepharose, gel filtration on Sephacryl S-300 and affinity chromatography on 2',5' ADP-Sepharose 4B colum
-
partial purification using column chromatography on DEAE-cellulose
-
partial, using ultrasonic oscillation, ammonium sulfate precipitation, column chromatography on Sephacryl S300 and DE-52 cellulose
-
recombuinant enzyme and isolated beta subunit
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Blue-Sepharose
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose and Sephadex G-200
-
using ammonium sulfate fractionation, column chromatography on DEAE-cellulose, Sepharose 6B, hydroxyapatite, glutamate dehydrogenase antibody affinity chromatography and column chromatography on Sephacryl S-200
-
using ammonium sulfate fractionation, coulmn chromatography on DEAE-cellulose, hydroxyapatite, Sepharose 6B and Sephadex G-200
-
using ammonium sulfate fractionation, gel filtration on Sephacryl S-300 and affinity chromatography on NADPH-Sepharose
-
using ammonium sulfate precipitation and column chromatography on DEAE-Sepharose, 2',5'-ADP-Sepharose and Sephacryl S-300
-
using heat treatment, ammonium sulfate precipitation, column chromatography on DEAE-Trisacryl, gel filtration and affinity chromatogray
-
using heat treatment, anion-exchange column chromatography on HiTrap Q and cation exchange column chromatography on Resources S
-
using protamine sulfate precipitation, ammonium sulfate precipitation, column chromatography on DE52, DEAE-Sephadex, hydroxyapatite, phenyl-Sepharose CL-4B and Bio-Gel filtration
-
using sonication, heat treatment, protamine sulfate precipitation, ammonium sulfate precipitation, gel filtration on G-50, column chromatography on DEAE-Sephadex and gel filtration on Sephadex G-200 and Sepharose 6B
-
using streptomycin sulfate treatment, ammonium sulfate fractionation, heat treatment, agarose gel filtration and DEAE-cellulose column chromatography
-
using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on DEAE-Sephacel, Bio-Gel A 1.5 m, Red Sepharose CL6B and Sephadex G-25
-
using streptomycin sulfate treatment, ammonium sulfate precipitation, column chromatography on Ultrogel AcA 22 and DEAE-Sephadex A-50, ultrafiltration with an Amicon PM 30 membrane and chromatography on hydroxyapatite column
-
using sulfate precipitation, gel filtration and column chromatography on 2',5'-ADP-Sepharose
-