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1.4.1.1: alanine dehydrogenase

This is an abbreviated version!
For detailed information about alanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.1

Reaction

L-alanine
+
H2O
 +
NAD+
=
pyruvate
 +
NH3
 +
NADH
 +
H+

Synonyms

(S)alanine dehydrogenase, (S)alanine:NAD oxidoreductase, 40 kDa antigen, ADH, AF1665, ALADH, alanine dehydrogenase, alanine oxidative deaminase, alanine oxidoreductase, ALD, AldA, alpha-alanine dehydrogenase, ApalaDH, AsAlaDH, CQA66_00465, dehydrogenase, alanine, glyoxylate reductive aminase, GxrA, HAADH1, HAADH2, L-Ala dehydrogenase, L-AlaDH, L-AlaDH-Bs, L-alanine dehydrogenase, MtbALD, NAD(H)-dependent L-alanine dehydrogenase, NAD+ dependent amino acid dehydrogenase, NAD+-dependent alanine dehydrogenase, NAD-dependent alanine dehydrogenase, NAD-linked alanine dehydrogenase, NADH-dependent alanine dehydrogenase, OF4Ald, PlaAlaDH, PvRA, pyruvate reductive aminase, Rv2780, ScALD, SCO1773, SheAlaDH, TB43

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.1 alanine dehydrogenase

General Stability

General Stability on EC 1.4.1.1 - alanine dehydrogenase

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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
6 M urea, complete inactivation and total loss of both alpha-helic and beta-structure
-
93% loss of activity after dialysis
-
absolute requirement of high ionic strength for stability
-
dialysis against 0.02 M Tris-HCl, pH 8.4, 75% loss of activity after 3 h, complete loss of activity after 24 h
-
enzyme immobilization using PEG causes 3-25% loss of activity
-
enzyme is extremely unstable and easily lysed
-
freezing and thawing completely destroys activity
-
inactivation after removal of salt
-
more than 75% loss of activity after two freeze-thaw cycles and storage at -20°C for 1 week, stabilized by addition of 50% glycerol
-
NaCl, required for stability, 2.0-4.3 M, 4°C, several weeks, stable
-
optimal KCl concentration for stability is 1.5 M
-
partial inactivation by dilution
-
RbCl and NaCl, significantly stabilize the enzyme. LiCl and KCl stabilize to a lower degree
repeated freezing and thawing leads to significant loss of activity
reversible deactivation by dilution
-
SDS, 10 min, 37°C, retains activity with up to 0.03% SDS, loss of activity with 0.05%
-
stable to repeated freezing and thawing
-
the purified enzyme is stabilized by EDTA, 2-mercaptoethanol, and DTT with effectivity in increasing order
Triton X-100, 10 min, 37°C, not inactivated by treatment with up to 4%
-
urea, above 3 M, denaturation of soluble enzyme, resistance to denaturation increases by immobilization, 5 M, 30 min, 85% loss of activity of matrix-bound enzyme
-