Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.3.99.41: 3-(methylsulfanyl)propanoyl-CoA 2-dehydrogenase

This is an abbreviated version!
For detailed information about 3-(methylsulfanyl)propanoyl-CoA 2-dehydrogenase, go to the full flat file.

Reaction

3-(methylsulfanyl)propanoyl-CoA
+
acceptor
=
3-(methylsulfanyl)acryloyl-CoA
+
reduced acceptor

Synonyms

3-methylmercaptopropionyl coenzyme A dehydrogenase, 3-methylmercaptopropionyl-CoA dehydrogenase, dmdC, DmdC1, MMPA-CoA dehydrogeanase, MMPA-CoA dehydrogenase, SAR11_0249, SL157_0694, SPO3804

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.41 3-(methylsulfanyl)propanoyl-CoA 2-dehydrogenase

Engineering

Engineering on EC 1.3.99.41 - 3-(methylsulfanyl)propanoyl-CoA 2-dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E435A
the mutation completely abolishes the activity of the enzyme
F195A
the mutation almost completely abolishes the activity of the enzyme to 0.4% of wild type activity
F287A
the mutant with 76.3% of wild type activity shows significantly increased Km to 3-(methylsulfanyl)propanoyl-CoA
H280A
the mutation severely reduces the activity of the enzyme to 18.2%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA
K223A
the mutation severely reduces the activity of the enzyme to 9.4%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA
K281A
the mutation severely reduces the activity of the enzyme to 54.5%, but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA
M161A
the mutation significantly decreases the activity of the enzyme to 37.5% and does not affect its Km value to FAD or 3-(methylsulfanyl)propanoyl-CoA
R284A
the mutant with 97.2% of wild type activity shows significantly increased Km to 3-(methylsulfanyl)propanoyl-CoA
R448A
the mutant with 44.9% of wild type activity shows significantly increased Km for 3-(methylsulfanyl)propanoyl-CoA
S197A
the mutation significantly decreases the activity of the enzyme to 3.6% and increases its Km value to FAD
T170A
the mutation significantly decreases the activity of the enzyme to 37.5% and increases its Km value to FAD
Y434A
the mutation significantly decreases the activity of the enzyme to 51.2% and increases its Km value to FAD but does not affect its Km value to 3-(methylsulfanyl)propanoyl-CoA