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(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
, 4,9(11)-androstadiene-3,17-dione + acceptor
? + reduced acceptor
11-deoxy-17alpha-hydroxycorticosterone + acceptor
17,21-dihydroxypregna-1,4-diene-3,20-dione + reduced acceptor
-
4-pregnene-17alpha,21-diol-3,20-dione
-
-
?
11-deoxycorticosterone + acceptor
21-hydroxypregna-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
11alpha-hydroxy-testosterone + acceptor
11alpha,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
-
?
11alpha-hydroxyprogesterone + acceptor
11alpha-hydroxy-1,4-pregnene-3,20-dione + reduced acceptor
-
4-pregnene-11alpha-ol-3,20-dione
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
11beta-cortisol + 2,6-dichlorophenol-indophenol
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
11beta-hydroxy-4-androstene-3,17-dione + acceptor
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
11beta-hydroxytestosterone + acceptor
11beta,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
4-androstene-11beta,17beta-diol-3-one
-
-
?
16,17alpha-epoxyprogesterone + acceptor
? + reduced acceptor
-
-
-
?
16alpha,17alpha-epoxyprogesterone + acceptor
? + reduced acceptor
17-methyl-testosterone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
17alpha,21-dihydroxyprogesterone 21-acetate + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
Reichstein substance S 21-acetate (RSA), best substrate
-
-
?
17alpha,21-dihydroxyprogesterone 21-acetate + acceptor
? + reduced acceptor
RSA
-
-
?
17alpha-hydroxy-progesterone + acceptor
1,4-pregnene-17alpha-ol-3,20-dione + reduced acceptor
-
4-pregnene-17alpha-ol-3,20-dione
-
-
?
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor
1,4-pregnene-17alpha-ol-3,20-dione + reduced acceptor
-
-
-
?
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor
? + reduced acceptor
17alpha-hydroxyprogesterone + 2,6-dichlorophenol-indophenol
17alpha-hydroxy-pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
17alpha-hydroxyprogesterone + acceptor
17alpha-hydroxy-pregna-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
17alpha-methyltestosterone + acceptor
17alpha-methylandrost-1,4-diene-3-one + reduced acceptor
-
-
-
-
?
19-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
19-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
19-nor-4-androstene-3,17-dione + acceptor
19-nor-1,4-androstene-3,17-dione + reduced acceptor
-
4-estren-3,17-dione
-
-
?
19-nortestosterone + acceptor
17beta-hydroxy-1,4-estrene-3-one + reduced acceptor
-
4-estren-17beta-ol-3-one
-
-
?
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor
? + reduced acceptor
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor
? + reduced acceptor
22-hydroxy-23, 24-bisnorchola-4-en-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
?
25-hydroxycholest-4-en-3-one + acceptor
? + reduced acceptor
-
-
-
?
3-oxo-5beta-cholan-24-oic acid + acceptor
? + reduced acceptor
-
-
-
-
?
3-oxosteroid + acceptor
3-oxo-DELTA1-steroid + reduced acceptor
-
key catalyst in microbial steroid metabolism
-
-
?
4-androsten-3-one-5-ene-17-carboxylic acid + acceptor
? + reduced acceptor
4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
4-androstene-3,11,17-trione + acceptor
1,4-androstene-3,11,17-trione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
4-androstene-3,17-dione + phenazine methosulfate
1,4-androstadiene-3,17-dione + reduced phenazine methosulfate
-
-
-
-
r
4-androstene-3,17-dione + phenazine methosulfate
1,4-androstene-3,17-dione + reduced phenazine methosulfate
-
-
-
-
?
4-cholesten-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-cholesten-3-one + 2,6-dichlorophenol-indophenol
? + reduced acceptor
4-cholesten-3-one + acceptor
? + reduced acceptor
-
-
-
?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
5alpha-androst-1-en-3-one + acceptor
5alpha-androstan-3-one + reduced acceptor
-
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
5beta-androstan-3,17-dione + acceptor
5beta-androst-1-en-3,17-dione + reduced acceptor
-
-
-
-
?
6-dehydrotestosterone acetate + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
9,22-dihydroxy-23,24-bisnorchol-4-ene-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
? + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + FAD
9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
androst-4,9(11)-diene-3,17-dione + acceptor
? + reduced acceptor
androst-4-ene-3,17-dione + acceptor
androsta-1,4-diene-3,17-dione + reduced acceptor
androsta-4,9(11)-diene-3,17-dione + acceptor
? + reduced acceptor
NSC 44826
-
-
?
androstanolone + acceptor
? + reduced acceptor
androsterone + acceptor
3-alpha-hydroxy-1-androstene-17-one + reduced acceptor
-
4-androstene-3,11,17-trione
-
-
?
canrenone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
best substrate
-
-
?
canrenone + acceptor
? + reduced acceptor
-
-
-
?
cortexolone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
corticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-17alpha,21-diol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
corticosterone + acceptor
1,4-pregnene-17alpha,21-diol-3,20-dione + reduced acceptor
-
4-pregnene-11beta,21-diol-3,20-dione
-
-
?
cortisol + acceptor
prednisolone + reduced acceptor
-
optimal concentration is 0.1 mg/ml, increase of concentration to 1 mg/ml reduces prednisolone yield
-
-
?
cortisone + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
cortisone + acceptor
? + reduced acceptor
cortisone + acceptor
prednisone + reduced acceptor
-
-
-
?
cortisone acetate + 2,6-dichlorophenol-indophenol
prednisolone acetate + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnadiene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
deoxycorticosterone + acceptor
1,4-pregnene-21-ol-3,20-dione + reduced acceptor
-
4-pregnene-21-ol-3,20-dione
-
-
?
dihydrotestosterone + acceptor
? + reduced acceptor
-
-
-
?
diosgenone + acceptor
? + reduced acceptor
hydrocorticosterone + acceptor
1,4-pregnene-11beta,17alpha,21-triol-3,20-dione + reduced acceptor
-
4-pregnene-11beta,17alpha,21triol-3,20-dione
-
-
?
hydrocortisone 21-hemisuccinate + acceptor
? + reduced acceptor
-
-
-
?
prednisolone + acceptor
? + reduced acceptor
-
-
-
?
pregn-4-ene-3,20-dione + acceptor
pregn-1,4-diene-3,20-dione + reduced acceptor
pregna-4,9(11),16-triene-3,20-dione + acceptor
? + reduced acceptor
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
progesterone + acceptor
? + reduced acceptor
-
-
-
-
?
progesterone + acceptor
pregna-1,4-diene-3,20-dione + reduced acceptor
spironolactone + acceptor
? + reduced acceptor
-
-
-
?
testololactone + acceptor
D-homo-17alpha-oxoandrosta-1,4-diene-3,17-one + reduced acceptor
-
D-homo-17a-oxoandrost-4-ene-3,17-dione
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
testosterone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
testosterone + acceptor
17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
testosterone propionate + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one propionate + reduced 2,6-dichlorophenol-indophenol
additional information
?
-
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
, 4,9(11)-androstadiene-3,17-dione + acceptor
? + reduced acceptor
-
-
-
-
?
, 4,9(11)-androstadiene-3,17-dione + acceptor
? + reduced acceptor
-
-
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + 2,6-dichlorphenolindophenol
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced 2,6-dichlorphenolindophenol
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione + phenazine methosulfate
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced phenazine methosulfate
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
hydrocortisone acetate
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
hydrocortisone acetate
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
hydrocortisone acetate
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
hydrocortisone
-
-
?
11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione acetate + reduced acceptor
hydrocortisone acetate
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
16alpha,17alpha-epoxyprogesterone + acceptor
? + reduced acceptor
-
-
-
-
?
16alpha,17alpha-epoxyprogesterone + acceptor
? + reduced acceptor
-
-
-
-
?
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor
? + reduced acceptor
-
-
-
?
17alpha-hydroxypregn-4-ene-3,20-dione + acceptor
? + reduced acceptor
-
-
-
?
19-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
19-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
19-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
19-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor
? + reduced acceptor
-
-
-
?
21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone + acceptor
? + reduced acceptor
-
-
-
?
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor
? + reduced acceptor
-
-
-
?
21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione + acceptor
? + reduced acceptor
-
-
-
?
22-hydroxy-23, 24-bisnorchola-4-en-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
22-hydroxy-23, 24-bisnorchola-4-en-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
?
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
?
-
-
-
?
4-androsten-3-one-5-ene-17-carboxylic acid + acceptor
? + reduced acceptor
-
-
-
?
4-androsten-3-one-5-ene-17-carboxylic acid + acceptor
? + reduced acceptor
-
-
-
?
4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
KSTD1, which is reported to be inactive with this substrates can catalyze the reaction if the solubility problem is addressed, e.g. by 2-hydroxpropyl-beta-cyclodextrin
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
ir
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
residues Y122, Y125, S138, E140, and Y541 from the FAD-binding domain and Y365 from the catalytic domain play a key role in this reaction
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
residues Y122, Y125, S138, E140, and Y541 from the FAD-binding domain and Y365 from the catalytic domain play a key role in this reaction
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
residues Y122, Y125, S138, E140, and Y541 from the FAD-binding domain and Y365 from the catalytic domain play a key role in this reaction
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-cholesten-3-one + 2,6-dichlorophenol-indophenol
? + reduced acceptor
-
-
-
?
4-cholesten-3-one + 2,6-dichlorophenol-indophenol
? + reduced acceptor
-
-
-
?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
6-dehydrotestosterone acetate + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
6-dehydrotestosterone acetate + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
preferred substrate of isozyme KSTD2
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + FAD
9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + FAD
9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
a 3-oxosteroid + acceptor
a 3-oxo-delta1-steroid + reduced acceptor
-
-
-
?
androst-4,9(11)-diene-3,17-dione + acceptor
? + reduced acceptor
-
-
-
?
androst-4,9(11)-diene-3,17-dione + acceptor
? + reduced acceptor
-
-
-
?
androst-4-ene-3,17-dione + acceptor
androsta-1,4-diene-3,17-dione + reduced acceptor
-
-
-
?
androst-4-ene-3,17-dione + acceptor
androsta-1,4-diene-3,17-dione + reduced acceptor
-
-
-
?
androstanolone + acceptor
? + reduced acceptor
-
-
-
?
androstanolone + acceptor
? + reduced acceptor
-
-
-
?
cholesterol + acceptor
?
-
KsdD2 exhibits low but detectable activity in the cholesterol degradation process
-
-
?
cholesterol + acceptor
?
-
KsdD2 exhibits low but detectable activity in the cholesterol degradation process
-
-
?
cortexolone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
cortexolone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
cortisone + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
4-pregnene-17alpha,21-diol-3,11,20-trione
-
-
?
cortisone + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
-
-
-
?
cortisone + acceptor
? + reduced acceptor
-
-
-
?
cortisone + acceptor
? + reduced acceptor
-
-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
diosgenone + acceptor
? + reduced acceptor
-
-
-
?
diosgenone + acceptor
? + reduced acceptor
-
-
-
?
pregn-4-ene-3,20-dione + acceptor
pregn-1,4-diene-3,20-dione + reduced acceptor
preferred substrate by synthetic recombinant enzyme DELTA1-KstDR
-
-
?
pregn-4-ene-3,20-dione + acceptor
pregn-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + acceptor
pregna-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
progesterone + acceptor
pregna-1,4-diene-3,20-dione + reduced acceptor
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + acceptor
17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
-
?
testosterone + acceptor
17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
-
?
testosterone + acceptor
17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
?
testosterone propionate + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one propionate + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone propionate + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one propionate + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: O2
-
-
?
additional information
?
-
-
3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5beta-steroids
-
-
?
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
enzyme works in an early step of steroid degradation
-
-
?
additional information
?
-
enzyme works in an early step of steroid degradation
-
-
?
additional information
?
-
-
KstD3 is active on a broad spectrum of substrates, comprising AD, progesterone, 4,9(11)-androstadiene-3,17-dione and 16alpha,17alpha-epoxyprogesterone. As usually the case with KstDs, the recombinant dehydrogenase is active neither with 3beta-/3alpha-hydroxysteroid nor with 3-ketosteroid containing a bulky C17-side chain, like 4-cholestene-3-one
-
-
-
additional information
?
-
-
KstD3 is active on a broad spectrum of substrates, comprising AD, progesterone, 4,9(11)-androstadiene-3,17-dione and 16alpha,17alpha-epoxyprogesterone. As usually the case with KstDs, the recombinant dehydrogenase is active neither with 3beta-/3alpha-hydroxysteroid nor with 3-ketosteroid containing a bulky C17-side chain, like 4-cholestene-3-one
-
-
-
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: O2
-
-
?
additional information
?
-
-
acceptor: O2
-
-
?
additional information
?
-
-
not: 3beta-hydroxysteroid, no electron acceptor: NADP+, K3Fe(CN)6, cytochrome c, 3-ketosteroids with a 11alpha- or 11beta-hydroxyl group are oxidized at slow rate, catalyzes aromatization of the A-ring of 19-nortestosterone and 19-norandrostenedione to produce estradiol and estrone, 19-hydroxytestosterone, 19-hydroxyandrosterone, 19-hydroxyandrostenedione and 19-oxotestosterone are converted to the respective phenolic steroids with cleavage of the C10 side-chain
-
-
?
additional information
?
-
-
the purified KstD catalyzes hydrogen transfer from 3-keto-4-ene-steroid (donor) into 3-keto-1,4-diene-steroid (acceptor), e.g. progesterone to 1,4-androstadiene-3,17-dione (ADD). The transhydrogenation reaction reaches equilibrium, and obeys to a typical ping-pong mechanism in which the donor is the first substrate, while the acceptor is the second. The transhydrogenation turnover number is of the same magnitude as that of the C1,2-dehydrogenation
-
-
-
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: menadione
-
-
?
additional information
?
-
-
acceptor: vitamin K2 (35), only 1-4% product formation as compared to 85-98% formation of 1,4-androstadiene-3,17-dione when phenazine methosulfate or menadione are used
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
-
-
?
additional information
?
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
enzyme KstD2 displays a high activity on a range of substrates, including 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-OH-P), androsta-4,9(11)-diene-3,17-dione (NSC 44826), and 4-androstene-3,17-dione (AD), substrate activity and kinetics, overview
-
-
-
additional information
?
-
-
enzyme KstD2 displays a high activity on a range of substrates, including 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-OH-P), androsta-4,9(11)-diene-3,17-dione (NSC 44826), and 4-androstene-3,17-dione (AD), substrate activity and kinetics, overview
-
-
-
additional information
?
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
among the three KstD isozymes, KstD2 shows the highest enzymatic activity when expressed heterogeneously, and KstD1 performs poorly, especially in Escherichia coli. For isozyme KstD3, the KstD enzyme activities are hardly detected in either host, Escherichia coli or Bacillus subtilis
-
-
-
additional information
?
-
enzyme KstD2 displays a high activity on a range of substrates, including 17alpha-hydroxypregn-4-ene-3,20-dione (17alpha-OH-P), androsta-4,9(11)-diene-3,17-dione (NSC 44826), and 4-androstene-3,17-dione (AD), substrate activity and kinetics, overview
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
the purified KstD from Mycobacterium smegmatis mc2155 displays high catalytic efficiency toward hydrocortisone, progesterone, and 9alpha-hydroxy-4-androstene-3,17-dione (9-OH-AD), and has highest affinity toward 9-OH-AD. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization. No or poor activity with 11beta,17-dihydroxy-6alpha-methyl-pregn-4-ene-3,20-dione and anecortave
-
-
-
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: menadione
-
-
?
additional information
?
-
-
substrate must have a 3-oxo-group, a DELTA4-double bond and axial hydrogens at 1alpha and 2beta positions
-
-
?
additional information
?
-
-
assay and detection with phenazine methosulfate (PMS) and 2,6-dichlorophenloindophenol (DCPIP). Catalytic activity and substrate profile of isozyme KsdD3, each KsdD isozyme exhibits distinct substrate profile, overview. KsdD3 is most active and versatile isozyme from Arthrobacter simplex. No activity of KsdD3 with 9alpha-hydroxy-4-androstene-3,17-dione, 17alpha-hydroxyprogesterone, 17alpha,21-dihydroxyprogesterone 21-acetate (RSA), dehydroepiandrosterone, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate, cortisone acetate, 5alpha-androstane-17beta-ol-3-one, and 5alpha-pregnane-3,20-dione
-
-
-
additional information
?
-
-
assay with phenazine methosulfate (PMS) and 2,6-dichlorophenloindophenol (DCPIP). Catalytic activity and substrate profile of isozyme KsdD5, each KsdD isozyme exhibits distinct substrate profile, overview. No activity of KsdD5 with 9alpha-hydroxy-4-androstene-3,17-dione, canrenone, 4-cholest-3-one, dehydroepiandrosterone, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate, 5alpha-androstane-17beta-ol-3-one, and 5alpha-pregnane-3,20-dione
-
-
-
additional information
?
-
-
assay and detection with phenazine methosulfate (PMS) and 2,6-dichlorophenloindophenol (DCPIP). Catalytic activity and substrate profile of isozyme KsdD3, each KsdD isozyme exhibits distinct substrate profile, overview. KsdD3 is most active and versatile isozyme from Arthrobacter simplex. No activity of KsdD3 with 9alpha-hydroxy-4-androstene-3,17-dione, 17alpha-hydroxyprogesterone, 17alpha,21-dihydroxyprogesterone 21-acetate (RSA), dehydroepiandrosterone, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate, cortisone acetate, 5alpha-androstane-17beta-ol-3-one, and 5alpha-pregnane-3,20-dione
-
-
-
additional information
?
-
-
assay with phenazine methosulfate (PMS) and 2,6-dichlorophenloindophenol (DCPIP). Catalytic activity and substrate profile of isozyme KsdD5, each KsdD isozyme exhibits distinct substrate profile, overview. No activity of KsdD5 with 9alpha-hydroxy-4-androstene-3,17-dione, canrenone, 4-cholest-3-one, dehydroepiandrosterone, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione, 11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate, 5alpha-androstane-17beta-ol-3-one, and 5alpha-pregnane-3,20-dione
-
-
-
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: menadione
-
-
?
additional information
?
-
-
acceptor: vitamin K2 (35), only 1-4% product formation as compared to 85-98% formation of 1,4-androstadiene-3,17-dione when phenazine methosulfate or menadione are used
-
-
?
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: menadione
-
-
?
additional information
?
-
-
acceptor: vitamin K2 (35), only 1-4% product formation as compared to 85-98% formation of 1,4-androstadiene-3,17-dione when phenazine methosulfate or menadione are used
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
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5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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in catalytic mechanism Tyr487 and Gly491 work in tandem to promote keto-enol tautomerization and increase the acidity of the C2 hydrogen atoms of the substrate. With assistance of Tyr119, the general base Tyr318 abstracts the axialbeta-hydrogen from C2 as a proton, whereas the FAD accepts the axial alpha-hydrogen from the C1 atom of the substrate as a hydride ion
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3-ketosteroid DELTA1-dehydrogenase (DELTA1-KstD) catalyzes the C-1 and C-2 desaturation of ring A. A direct-coupled flurometric assay is developed based on resazurin, which is a weakly fluorescent redox dye that is irreversibly reduced when it accepts protons released from a donor molecule. Reduction of resazurin results in the formation of the highly fluorescent product, resorufin. In this assay, DELTA1-KstDR removes and donates two protons and two electrons from C-1 and C-2 of ring-A directly to resazurin, resulting in the formation of resorufin and water. This reduction can be measured by monitoring the increase in fluorescence intensity with time, allowing the assessment of the initial rates of DELTA1-KstDR substrate conversion. Substrate specificity analysis of the recombinant enzyme DELTA1-KstDR, overview. Poor or no activity with mifepristone, cholesterol, pregnenolone, dehydroepiandrosterone, corticosterone, 7beta-hydroxycholestenone, prednisolone, cholestenone, and aldosterone
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additional information
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enzyme substrate specificity, structure-function analysis. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. Enzyme KSTD1 prefers smaller 3-oxosteroids
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additional information
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enzyme substrate specificity, structure-function analysis. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. Enzyme KSTD1 prefers smaller 3-oxosteroids
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additional information
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KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization
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additional information
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KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization
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additional information
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KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization
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additional information
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4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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in catalytic mechanism Tyr487 and Gly491 work in tandem to promote keto-enol tautomerization and increase the acidity of the C2 hydrogen atoms of the substrate. With assistance of Tyr119, the general base Tyr318 abstracts the axialbeta-hydrogen from C2 as a proton, whereas the FAD accepts the axial alpha-hydrogen from the C1 atom of the substrate as a hydride ion
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additional information
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5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
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additional information
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KstD from Rhodococcus erythropolis WY 1406 exhibits high catalytic efficiency toward androst-4,9(11)-diene-3,17-dione, 21-acetoxy-pregna-4,9(11),16-triene-3,20-dione, and cortexolone, where in all three cases the Km values are 2.5-4fold lower than that toward hydrocortisone. 4-Androstene-3,17-dione is a good substrate. Substrate specificity, overview. Assay method evaluation and optimization
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acceptor: phenazine methosulfate
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acceptor: quinones
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no electron acceptor: NAD+, NADP+
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3-ketosteroid DELTA1-dehydrogenase (DELTA1-KstD) catalyzes the C-1 and C-2 desaturation of ring A
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enzyme substrate specificity, structure-function analysis. Conversion of 4-androstene-3,17-dione and cholest-4-en-3-one by isozyme AcmB2 proceeds at a similar rate. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. AcmB2 shows ability to catalyze DELTA1-dehydrogenation of steroids with the aliphatic side chain on the C17 position
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additional information
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enzyme substrate specificity, structure-function analysis. Conversion of 4-androstene-3,17-dione and cholest-4-en-3-one by isozyme AcmB2 proceeds at a similar rate. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. AcmB2 shows ability to catalyze DELTA1-dehydrogenation of steroids with the aliphatic side chain on the C17 position
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additional information
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enzyme substrate specificity, structure-function analysis. Conversion of 4-androstene-3,17-dione and cholest-4-en-3-one by isozyme AcmB2 proceeds at a similar rate. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. AcmB2 shows ability to catalyze DELTA1-dehydrogenation of steroids with the aliphatic side chain on the C17 position
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additional information
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enzyme substrate specificity, structure-function analysis. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. AcmB shows ability to catalyze DELTA1-dehydrogenation of steroids with the aliphatic side chain on the C17 position. AcmB indeed exhibits a significantly higher affinity to cholest-4-en-3-one than to 4-androstene-3,17-dione
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additional information
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enzyme substrate specificity, structure-function analysis. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. AcmB shows ability to catalyze DELTA1-dehydrogenation of steroids with the aliphatic side chain on the C17 position. AcmB indeed exhibits a significantly higher affinity to cholest-4-en-3-one than to 4-androstene-3,17-dione
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additional information
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enzyme substrate specificity, structure-function analysis. KSTDs catalyze regio- and stereoselective dehydrogenation between C1 and C2 atoms of steroid ring A. AcmB shows ability to catalyze DELTA1-dehydrogenation of steroids with the aliphatic side chain on the C17 position. AcmB indeed exhibits a significantly higher affinity to cholest-4-en-3-one than to 4-androstene-3,17-dione
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additional information
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the recombinant enzyme is active with various 3-ketosteroids, including exceptionally the bulky side chain-containing steroids, 4-cholesten-3-one and 25-hydroxycholest-4-en-3-one
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