1.3.99.4: 3-oxosteroid 1-dehydrogenase

This is an abbreviated version!
For detailed information about 3-oxosteroid 1-dehydrogenase, go to the full flat file.

Word Map on EC 1.3.99.4

Reaction

a 3-oxosteroid
+
acceptor
=
a 3-oxo-DELTA1-steroid
+
reduced acceptor

Synonyms

1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase, 4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase, 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase, 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase, D1-dehydrogenase, D1-steroid reductase, dehydrogenase, 3-oxo steroid Delta 1-, Delta1-dehydrogenase, DELTA1-KSTD1, DELTA1-steroid reductase, ketosteroid DELTA1-dehydrogenase, KsdD, KsdD1, KsdD2, KsdDM, KSDH, KstD, KSTD1, KSTD2, KSTD3, KSTD3 orthologue, KstDF, Rv3537, steroid-1-dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.4 3-oxosteroid 1-dehydrogenase

Reference

Reference on EC 1.3.99.4 - 3-oxosteroid 1-dehydrogenase

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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Itagaki, E.; Wakabayashi, T.; Hatta, T.
Purification and characterization of 3-ketosteroid-delta 1-dehydrogenase from Nocardia corallina
Biochim. Biophys. Acta
1038
60-67
1990
Gordonia rubripertincta
Manually annotated by BRENDA team
Atrat, P.; Deppmeyer, V.; Hörhold, C.
Efficient purification of a microbial steroid-1-dehydrogenase by electrophoretic desorption from the affinity matrix on a preparative scale
J. Chromatogr.
189
279-283
1980
Rhodococcus opacus
-
Manually annotated by BRENDA team
Groh, H.; Komel, R.; Deppmeyer, V.; Atrat, P.; Hörhold, C.
Steroid-transforming enzymes from microorganisms. XI. Reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from Nocardia opaca
Z. Allg. Mikrobiol.
19
727-730
1979
Rhodococcus opacus
Manually annotated by BRENDA team
Itagaki, E.; Hatta, T.; Wakabayashi, T.; Suzuki, K.
Spectral properties of 3-ketosteroid-delta 1-dehydrogenase from Nocardia corallina
Biochim. Biophys. Acta
1040
281-286
1990
Gordonia rubripertincta
Manually annotated by BRENDA team
Kloosterman IV, J.; Lilly, M.D.
Effect of supersaturated aqueous hydrocortison concentrations on the DELTA1-dehydrogenase activity of free and immobilized Arthrobacter simplex
Enzyme Microb. Technol.
6
113-116
1984
Pimelobacter simplex
-
Manually annotated by BRENDA team
Penasse, L.; Nomine, G.
The active center of the 3-oxosteroid delta-1-dehydrogenase from Arthrobacter simplex
Eur. J. Biochem.
47
555-559
1974
Pimelobacter simplex
Manually annotated by BRENDA team
Aries, V.C.; Goddard, P.; Hill, M.J.
Degradation of steroids by intestinal bacteria. III. 3-Oxo-5beta-steroid DELTA1-dehydrogenase and 3-oxo-5beta-steroid DELTA4-dehydrogenase
Biochim. Biophys. Acta
248
482-488
1971
Clostridium paraputrificum
-
Manually annotated by BRENDA team
Rodig, O.R.; Roller, P.P.; Nicholas, A.W.
The stereochemistry of hydrogen addition by a delta1-steroid reductase in bakers yeast
Biochem. Biophys. Res. Commun.
56
467-470
1974
Saccharomyces cerevisiae
Manually annotated by BRENDA team
Levy, H.R.; Talalay, P.
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation
J. Biol. Chem.
234
2014-2021
1959
Comamonas testosteroni
Manually annotated by BRENDA team
Atrat, P.; Groh, H.
Steroid transformation with immobilized microorganisms. VI. The reverse reaction of steroid-1-dehydrogenases from different micoorganisms in immobilized state
Z. Allg. Mikrobiol.
21
3-6
1981
Mycolicibacterium phlei, Nocardia erythropolis, Rhodococcus opacus
Manually annotated by BRENDA team
Udvardy, E.N.; Hantos, G.
Kinetics of the formation of steroid-specific enzymes
FEMS Symp.
13
21-33
1982
Pimelobacter simplex
-
Manually annotated by BRENDA team
Hörhold, C.; Atrat, P.; Deppmeyer, V.; Gabert, A.; Flemming, C.; Schubert, K.
Steroid-transforming enzymes from microorganisms. II. Purification of a 3-oxosteroid: (2,6-dichlorophenolindophenol) delta 1-oxidoreductase from Nocardia opaca by means of affinity chromatography
Z. Allg. Mikrobiol.
16
559-561
1976
Rhodococcus opacus
Manually annotated by BRENDA team
Lestrovaja, N.N.; Dänhardt, S.; Hörhold, C.
Steroid transforming enzymes from microorganisms. V. Purification of a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca and characterization of the prosthetic group
Z. Allg. Mikrobiol.
18
189-196
1978
Rhodococcus opacus
Manually annotated by BRENDA team
Hörhold, C.; Huller, E.; Rose, G.
Steroid-transforming enzymes from microorganisms. XII. Induction characteristics of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase in Nocardia opaca
Z. Allg. Mikrobiol.
19
731-739
1979
Rhodococcus opacus
Manually annotated by BRENDA team
Hörhold, C.; Huller, E.; Rose, G.
Steroid transforming enzymes in microorganisms. XIII. Relationship between steroid structure and induction of 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in Nocardia opaca
Z. Allg. Mikrobiol.
20
23-32
1980
Rhodococcus opacus
Manually annotated by BRENDA team
Abul-Hajj, Y.J.
Isolation of vitamin K2(35) from Nocardia restrictus and Corynebacterium simplex. A natural electron acceptor in microbial steroid ring A dehydrogenations
J. Biol. Chem.
253
2356-2360
1978
Ilyonectria destructans, Pimelobacter simplex, Plectosphaerella cucumerina, Rhodococcus hoagii
Manually annotated by BRENDA team
Van der Geize, R.; Hessels, G.I.; van Gerwen, R.; van der Meijden, P.; Dijkhuizen, L.
Unmarked gene deletion mutagenesis of kstD, encoding 3-ketosteroid DELTA1-dehydrogenase, in Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker
FEMS Microbiol. Lett.
205
197-202
2001
Rhodococcus erythropolis
Manually annotated by BRENDA team
Choi, K.P.; Molnar, I.; Yamashita, M.; Murooka, Y.
Purification and characterization of the 3-ketosteroid-DELTA1-dehydrogenase of Arthrobacter simplex produced in Streptomyces lividans
J. Biochem.
117
1043-1049
1995
Pimelobacter simplex
Manually annotated by BRENDA team
Choi, K.P.; Molnar, I.; Murooka, Y.
Secretory overproduction of Arthrobacter simplex 3-ketosteroid DELTA1-dehydrogenase by Streptomyces lividans with a multi-copy shuttle vector
Appl. Microbiol. Biotechnol.
43
1044-1049
1995
Pimelobacter simplex
Manually annotated by BRENDA team
Fujii, C.; Morii, S.; Kadode, M.; Sawamoto, S.; Iwami, M.; Itagaki, E.
Essential tyrosine residues in 3-ketosteroid-DELTA1-dehydrogenase from Rhodococcus rhodochrous
J. Biochem.
126
662-667
1999
Rhodococcus rhodochrous
Manually annotated by BRENDA team
Molnar, I.; Choi, K.P.; Yamashita, M.; Murooka, Y.
Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-DELTA1-dehydrogenase, 3-ketosteroid-DELTA5-isomerase and a hypothetical regulatory protein
Mol. Microbiol.
15
895-905
1995
Pimelobacter simplex
Manually annotated by BRENDA team
Morii, S.; Fujii, C.; Miyoshi, T.; Iwami, M.; Itagaki, E.
3-Ketosteroid-DELTA1-dehydrogenase of Rhodococcus rhodochrous: sequencing of the genomic DNA and hyperexpression, purification, and characterization of the recombinant enzyme
J. Biochem.
124
1026-1032
1998
Rhodococcus rhodochrous
Manually annotated by BRENDA team
Horinouchi, M.; Hayashi, T.; Yamamoto, T.; Kudo, T.
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes
Appl. Environ. Microbiol.
69
4421-4430
2003
Comamonas testosteroni (Q06401), Comamonas testosteroni TA441 (Q06401)
Manually annotated by BRENDA team
van der Geize, R.; Hessels, G.I.; Dijkhuizen, L.
Molecular and functional characterization of the kstD2 gene of Rhodococcus erythropolis SQ1 encoding a second 3-ketosteroid DELTA(1)-dehydrogenase isoenzyme
Microbiology
148
3285-3292
2002
Rhodococcus erythropolis (Q8RLT5), Rhodococcus erythropolis SQ1 (Q8RLT5), Rhodococcus erythropolis SQ1
Manually annotated by BRENDA team
Adham, N.Z.; El-Hady, A.A.; Naim, N.
Biochemical studies on the microbial DELTA1-dehydrogenation of cortisol by Pseudomonas fluorescens
Proc. Biochem.
38
897-902
2003
Pseudomonas fluorescens
-
Manually annotated by BRENDA team
Brzostek, A.; Sliwinski, T.; Rumijowska-Galewicz, A.; Korycka-Machala, M.; Dziadek, J.
Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis
Microbiology
151
2393-2402
2005
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Ra / ATCC 25177, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
Manually annotated by BRENDA team
Knol, J.; Bodewits, K.; Hessels, G.I.; Dijkhuizen, L.; van der Geize, R.
3-Keto-5alpha-steroid DELTA(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism
Biochem. J.
410
339-346
2008
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv, Rhodococcus erythropolis (A9QAE5), Rhodococcus erythropolis (Q8RLT5), Rhodococcus erythropolis (Q9RA02), Rhodococcus erythropolis, Rhodococcus erythropolis SQ1 (A9QAE5), Rhodococcus erythropolis SQ1 (Q8RLT5), Rhodococcus erythropolis SQ1 (Q9RA02), Rhodococcus erythropolis SQ1
Manually annotated by BRENDA team
Li, Y.; Lu, F.; Sun, T.; Du, L.
Expression of ksdD gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis
Lett. Appl. Microbiol.
44
563-568
2007
Pimelobacter simplex
Manually annotated by BRENDA team
Rohman, A.; van Oosterwijk, N.; Dijkstra, B.W.
Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid DELTA1-dehydrogenase from Rhodococcus erythropolis SQ1
Acta Crystallogr. Sect. F
68
551-556
2012
Rhodococcus erythropolis (Q9RA02), Rhodococcus erythropolis SQ1 (Q9RA02)
Manually annotated by BRENDA team
Wei, W.; Wang, F.Q.; Fan, S.Y.; Wei, D.Z.
Inactivation and augmentation of the primary 3-ketosteroid-DELTA1-dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione
Appl. Environ. Microbiol.
76
4578-4582
2010
Mycolicibacterium neoaurum (C6KID9), Mycolicibacterium neoaurum NwIB-01 (C6KID9)
Manually annotated by BRENDA team
Chen, M.M.; Wang, F.Q.; Lin, L.C.; Yao, K.; Wei, D.Z.
Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-DELTA1-dehydrogenase in steroid transformation
Appl. Microbiol. Biotechnol.
96
133-142
2012
Aspergillus fumigatus, Aspergillus fumigatus CICC 40167
Manually annotated by BRENDA team
Brzezinska, M.; Szulc, I.; Brzostek, A.; Klink, M.; Kielbik, M.; Sulowska, Z.; Pawelczyk, J.; Dziadek, J.
The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis
BMC Microbiol.
13
43
2013
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
Manually annotated by BRENDA team
Zhang, W.; Shao, M.; Rao, Z.; Xu, M.; Zhang, X.; Yang, T.; Li, H.; Xu, Z.
Bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Mycobacterium neoaurum JC-12
J. Steroid Biochem. Mol. Biol.
135
36-42
2013
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
Manually annotated by BRENDA team
Rohman, A.; van Oosterwijk, N.; Thunnissen, A.M.; Dijkstra, B.W.
Crystal structure and site-directed mutagenesis of 3-ketosteroid Delta1-dehydrogenase from Rhodococcus erythropolis SQ1 explain its catalytic mechanism
J. Biol. Chem.
288
35559-35568
2013
Rhodococcus erythropolis (Q9RA02), Rhodococcus erythropolis SQ1 (Q9RA02)
Manually annotated by BRENDA team
Shao, M.; Zhang, X.; Rao, Z.; Xu, M.; Yang, T.; Li, H.; Xu, Z.; Yang, S.
A mutant form of 3-ketosteroid-Delta(1)-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095
J. Ind. Microbiol. Biotechnol.
43
691-701
2016
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12, Mycolicibacterium neoaurum ST-095
Manually annotated by BRENDA team
Yao, K.; Xu, L.Q.; Wang, F.Q.; Wei, D.Z.
Characterization and engineering of 3-ketosteroid-Delta1-dehydrogenase and 3-ketosteroid-9alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols
Metab. Eng.
24
181-191
2014
Mycolicibacterium neoaurum (C8CJG6), Mycolicibacterium neoaurum (W0NR13), Mycolicibacterium neoaurum (W0NUN5), Mycolicibacterium neoaurum ATCC 25795 (C8CJG6), Mycolicibacterium neoaurum ATCC 25795 (W0NR13), Mycolicibacterium neoaurum ATCC 25795 (W0NUN5)
Manually annotated by BRENDA team
Guevara, G.; Fernandez de Las Heras, L.; Perera, J.; Navarro Llorens, J.M.
Functional differentiation of 3-ketosteroid Delta(1)-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4
Microb. Cell Fact.
16
42
2017
Rhodococcus ruber (C6GCU2), Rhodococcus ruber (I0B6I6), Rhodococcus ruber (I0B6J0), Rhodococcus ruber, Rhodococcus ruber Chol-4 (C6GCU2), Rhodococcus ruber Chol-4 (I0B6I6), Rhodococcus ruber Chol-4 (I0B6J0)
Manually annotated by BRENDA team