1.3.99.4: 3-oxosteroid 1-dehydrogenase

This is an abbreviated version!
For detailed information about 3-oxosteroid 1-dehydrogenase, go to the full flat file.

Word Map on EC 1.3.99.4

1.3.99.4

rhodococcus

3-ketosteroids

mycobacterium

cholesterol

4-androstene-3,17-dione

erythropolis

synthesis

arthrobacter

simplex

isoenzymes

testosteroni

rhodochrous

androst-4-ene-3,17-dione

bioconversion

hydrocortisone

dehydrogenation

phytosterols

ruber

steroid-transforming

globiformis

1,4-androstadiene-3,17-dione

neoaurum

molecular biology

Reaction

Synonyms

1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenases, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase, 4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase, 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase, 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase, AcmB, AcmB2, cholest-4-en-3-one DELTA1-dehydrogenase, D1-dehydrogenase, D1-steroid reductase, dehydrogenase, 3-oxo steroid Delta 1-, Delta1-dehydrogenase, DELTA1-KstD, DELTA1-KSTD1, DELTA1-KstDA, DELTA1-KstDR, DELTA1-steroid reductase, ketosteroid DELTA1-dehydrogenase, KsdD, KsdD1, KsdD2, KsdD3, KsdD5, KsdDM, KSDH, KstD, KSTD1, KSTD2, KSTD3, KSTD3 orthologue, KstDF, MnKstD1, MnKstD2, MsKstD1, MsKstD2, MsKstD3, MsKstD4, ReKstD1, Rv3537, steroid-1-dehydrogenase

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.99 With unknown physiological acceptors

1.3.99.4 3-oxosteroid 1-dehydrogenase

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Results	in table
11	Activating Compound
1430	AA Sequence
20	Application
1	CAS Registry Number
36	Cloned(Commentary)
24	Cofactor
2	Crystallization (Commentary)
4	Expression
42	General Information
5	General Stability
42	Inhibitors
45	kcat/KM [mM/s]
135	KM Value [mM]
9	Localization
9	Metals/Ions
10	Molecular Weight [Da]
65	Natural Substrates/ Products (Substrates)
1	Organic Solvent Stability
547	Organism
8	Pathway
18	PDB ID
24	pH Optimum
4	pH Range
5	pH Stability
3	pI Value
58	Protein Variants
19	Purification (Commentary)
4	Reaction
3	Reaction Type
107	Reference
31	Specific Activity [micromol/min/mg]
4	Storage Stability
341	Substrates and Products (Substrate)
32	Subunits
232	Synonyms
1	Systematic Name
18	Temperature Optimum [°C]
3	Temperature Range [°C]
10	Temperature Stability [°C]
56	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 1.3.99.4 - 3-oxosteroid 1-dehydrogenase

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1084.2 - 1503.7

11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione

2 entries

769.7 - 875

11beta,17alpha,21-trihydroxypregn-4-ene-3,20-dione acetate

2 entries

513 - 675.2

17-methyl-testosterone

2 entries

834

17alpha,21-dihydroxyprogesterone 21-acetate

Pimelobacter simplex

pH 7.5, 35°C, recombinant isozyme KsdD3

763180

1175.8 - 1203.9

17alpha-hydroxypregn-4-ene-3,20-dione

2 entries

597.9

17alpha-hydroxyprogesterone

Pimelobacter simplex

pH 7.5, 35°C, recombinant isozyme KsdD3

763180

367.3 - 1511.2

21-acetoxy-pregna-4,9(11),16-triene-3,20-dinone

2 entries

644.7 - 663.2

21-chloro-17-hydroxy-pregna-4,9(11)-diene-3,20-dione

2 entries

165.7 - 447.7

4-androsten-3-one-5-ene-17-carboxylic acid

2 entries

0.108 - 1838

4-androstene-3,17-dione

10 entries

420.4 - 1590.8

9alpha-hydroxy-4-androstene-3,17-dione

2 entries

48.1 - 1592.9

androst-4,9(11)-diene-3,17-dione

2 entries

1144.6

canrenone

Pimelobacter simplex

pH 7.0, 30°C, recombinant isozyme KsdD3

763180

1009.6 - 1674.8

cortexolone

2 entries

6 - 904.4

cortisone

2 entries

759

cortisone acetate

Pimelobacter simplex

pH 7.5, 35°C, recombinant isozyme KsdD3

763180

192

pregna-4,9(11),16-triene-3,20-dione

Mycolicibacterium smegmatis

A0QW99, A0R1T2, A0R1T7

pH 8.0, 25°C

762654

263.4 - 1916

progesterone

4 entries

341 - 1141.6

testosterone

4 entries