3-oxosteroid 1-dehydrogenase

This is an abbreviated version!
For detailed information about 3-oxosteroid 1-dehydrogenase, go to the full flat file.

Word Map on EC


a 3-oxosteroid
a 3-oxo-DELTA1-steroid
reduced acceptor


1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase, 4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase, 4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase, 4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase, D1-dehydrogenase, D1-steroid reductase, dehydrogenase, 3-oxo steroid Delta 1-, Delta1-dehydrogenase, DELTA1-KSTD1, DELTA1-steroid reductase, ketosteroid DELTA1-dehydrogenase, KsdD, KsdD1, KsdD2, KsdDM, KSDH, KstD, KSTD1, KSTD2, KSTD3, KSTD3 orthologue, KstDF, Rv3537, steroid-1-dehydrogenase


     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
       3-oxosteroid 1-dehydrogenase


Cloned on EC - 3-oxosteroid 1-dehydrogenase

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CLONED (Commentary)
expressed in Bacillus subtilis strain WB600
expressed in Escherichia coli BL21(DE3) cells
expression in Escherichia coli
expression in Streptomyces lividans
gene ksdD, phylogenetic analysis, expression in Bacillus subtilis strain 168 with 18fold higher activity compared to wild-type Mycobacterium neoaurum strain JC-12
gene kstD, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression of the His-tagged enzyme, by low-temperature induction at 22°C, the recombinant protein appears partly as soluble protein
gene kstD1, heterologous expression of the His-tagged DELTA1-KSTD1 protein in Escherichia coli strain BL21(DE3)
gene kstDF, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21(lambdaDE3) and in Pichia pastoris strains KM71 and GS115 cytoplasm, both intracellularly and extracelluarly, the latter is inactive, method optimization
into the pJam2 expression vector under the acetamidase promoter and introduced into the deltaksdD-1 mutant
site directed mutagenesis of steroid binding site, Y-121 involved in catalytic function, Y-116, Y-104 involved in substrate binding
under control of the Phsp promoter and introduced into mutant cells of Mycobacterium smegmatis
unmarked gene deletion, biochemical evidence for a second 3-ketosteroid-DELTA1-dehydrogenase