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1.3.99.29: phytoene desaturase (zeta-carotene-forming)

This is an abbreviated version!
For detailed information about phytoene desaturase (zeta-carotene-forming), go to the full flat file.

Word Map on EC 1.3.99.29

Reaction

15-cis-phytoene
+ 2 acceptor =
all-trans-zeta-carotene
+ 2 reduced acceptor

Synonyms

4-step phytoene desaturase, bacterial-type phytoene desaturase, crtI-type phytoene desaturase, Crtla, glr0867, PDS, phytoene desaturase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.29 phytoene desaturase (zeta-carotene-forming)

Cloned

Cloned on EC 1.3.99.29 - phytoene desaturase (zeta-carotene-forming)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene pds, DNA and amino acid sequence determination and analysis of wild-type and mutant E17, expression of wild-type and mutants in Escherichia coli
gene pds, DNA and amino acid sequence determination and analysis, phylogenetic analysis, functional expression in Escherichia coli strain JM109 or BL21(DE3), subcloning in Escherichia coli strain DH5alpha
gene pds, DNA and amino acid sequence determination and analysis, sequence comarisons
gene pds, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, expression of wild-type and mutant enzymes in Escherichia coli strains JM109 and BL21(DE3), quantitative real time PCR expression assay
when glr0867 is expressed in Escherichia coli, lycopene accumulates as the major pigment, and intermediates (asymmetric-zeta-carotene and neurosporene) are also detected