1.3.99.28: phytoene desaturase (neurosporene-forming)
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For detailed information about phytoene desaturase (neurosporene-forming), go to the full flat file.
Reaction
Synonyms
3-step phytoene desaturase, CrtI, PDS, phytoene desaturase
ECTree
1.3.99.28 phytoene desaturase (neurosporene-forming)Advanced search results
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results (Cloned
Cloned on EC 1.3.99.28 - phytoene desaturase (neurosporene-forming)
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CLONED (Commentary) 
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene crtI, cloning and expression of wild-type and mutant enzymes in Escherichia coli strain DH5alpha
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gene crtI, cloning of the crt gene cluster crtCDEF, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
gene crtI, sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
sequence comparisons and phylogenetic analysis, recombinant expression in Escherichia coli
the phytoene desaturase gene, from Rhodobacter capsulatus is functionally complemented with a gene construct from Erwinia uredovora which encodes all enzymes responsible for formation of 15-cis phytoene in Escherichia coli
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gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
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gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
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gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
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gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
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gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
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gene crtI, phylogenetic tree, co-expression with 5 other enzymes of the carotenoid pathway from Pantoea agglomerans, i.e. IPP isomerase, FPP synthase, GGPP synthase, phytoene synthase, lycopene cyclase, beta-carotene hydrolase, and zeaxanthin glucosyltransferase, in Escherichia coli, functional complementation by CrtI of Brevibacterium linens, CGI, CrtI of Corynebacterium glutamicum, RSI, CrtI of Rhodobacter sphaeroides RCI, CrtI of Rhodobacter capsulatus, and RBI, CrtI of Rhodopirellula baltica, and the homologous complementation of CrtI from Pantoea agglomerans with the Pantoea agglomerans carotenogenic module expressing CrtEPAG -CrtBPAG
