1.3.99.17: quinoline 2-oxidoreductase

This is an abbreviated version!
For detailed information about quinoline 2-oxidoreductase, go to the full flat file.

Word Map on EC 1.3.99.17

Reaction

Quinoline
+
acceptor
+
H2O
=
quinolin-2(1H)-one
+
reduced acceptor

Synonyms

Dehydrogenase, quinoline 2-, Quinoline oxidoreductase, Qor, QuinOr, quinoline 2-oxidoreductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.99 With unknown physiological acceptors
                1.3.99.17 quinoline 2-oxidoreductase

Engineering

Engineering on EC 1.3.99.17 - quinoline 2-oxidoreductase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
E743D
-
about twofold increase in KM-value for quinoline, 150fold reduction of kcat-value
E743V
-
about twofold increase in KM-value for quinoline, drastic reduction of kcat-value
F375V
-
as stable as wild-type
A259G
-
turnover rate 2.4fold reduced, no significant altered Km
W331G
-
turnover rate 38fold reduced, suitable for hydroxylation of quinoxaline and C4-substituted quinolines
V373A
-
turnover rate 40fold reduced
V373Y
-
as stable as wild-type
A546G
-
turnover rate 199fold reduced, increased affinity for quinoline and quinoxaline
E743D
-
about twofold increase in KM-value for quinoline, 150fold reduction of kcat-value
-
E743V
-
about twofold increase in KM-value for quinoline, drastic reduction of kcat-value
-
F375V
Pseudomonas putida 86-1deltaqor
-
as stable as wild-type
-
A259G
Pseudomonas putida 86-1deltaqor
-
turnover rate 2.4fold reduced, no significant altered Km
-
W331G
Pseudomonas putida 86-1deltaqor
-
turnover rate 38fold reduced, suitable for hydroxylation of quinoxaline and C4-substituted quinolines
-
V373A
Pseudomonas putida 86-1deltaqor
-
turnover rate 40fold reduced
-
V373Y
Pseudomonas putida 86-1deltaqor
-
as stable as wild-type
-
additional information