1.3.98.3: coproporphyrinogen dehydrogenase

This is an abbreviated version!
For detailed information about coproporphyrinogen dehydrogenase, go to the full flat file.

Word Map on EC 1.3.98.3

Reaction

Coproporphyrinogen III
+ 2 S-adenosyl-L-methionine =
protoporphyrinogen IX
+ 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine

Synonyms

anaerobic coproporphyrinogen III oxidase, At5g63290, AtHEMN1, BtrN, Coprogen oxidase, coproporphyrinogen III oxidase, coproporphyrinogen oxidase, coproporphyrinogenase, CPO, HemN, HEMN1, HemW, More, oxidase, coproporphyrinogen, oxygen-independent coproporphyrinogen III oxidase, oxygen-independent coproporphyrinogen-III oxidase, oxygen-independent CPO, radical S-adenosyl-L-methionine dehydrogenase, radical SAM enzyme, Sll1876, Sll1917, viperin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.98 With other, known, physiological acceptors
                1.3.98.3 coproporphyrinogen dehydrogenase

Cloned

Cloned on EC 1.3.98.3 - coproporphyrinogen dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
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expressed in Escherichia coli BL21 cells
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expressed in Escherichia coli strain BL21(DE3)
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gene cig5, expression of viperin lacking the leucine zipper domain, viperin43-340, in Escherichia coli Rosetta(DE3)pLysS cells
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gene hemN, expression in Escherichia coli strain BL21(DE3)
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gene HEMN1, promoter and transcript analyses of AtHEMN1, recombinant expression of AtHEMN1-GFP fusion protein in mitochondria, quantitative reverse transcription PCR enzyme expression analysis
gene hemW or hemN, recombinant expression in the hemN-deficient Escherichia coli mutant strain JW3838 and in strain BL21(DE3) as His-tagged protein. Lactococcus lactis HemW at high levels does not complement the growth deficit of the Escherichia coli DELTAhemN mutant
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genes sll1876 and sll1917, overexpression of N-terminally Strep-tagged HemNs in Escherichia coli
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hemeN gene, overexpression in Escherichia coli BL21(DE3)
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mutated enzymes expressed in Escherichia coli BL21(DE3)
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recombinant expression as GST-fusion protein
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viperin from monocytic cells, DNA and amino acid sequence determination and analysis, expression of His-tagged full-length enzyme, and of His-tagged viperin (45-361) and viperin (214-361) in Escherichia coli strain BL21
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