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1.3.8.8: long-chain acyl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about long-chain acyl-CoA dehydrogenase, go to the full flat file.

Word Map on EC 1.3.8.8

Reaction

a long-chain acyl-CoA
+
electron-transfer flavoprotein
=
a long-chain trans-2,3-dehydroacyl-CoA
 +
reduced electron-transfer flavoprotein

Synonyms

ACAD 9, AcdB, acyl-CoA dehydrogenase 9, BACH, CTE-II, EC 1.3.99.13, EC 1.3.99.3, LCAD, long chain acyl coenzyme A dehydrogenase, long chain acyl-CoA dehydrogenase, long-chain acyl-CoA dehydrogenase, long-chain acyl-CoA hydrolase, long-chain acyl-coenzyme A dehydrogenase, MCAD, medium long-chain acyl-CoA dehydrogenase, More, MTE-II, palmitoyl-CoA dehydrogenase, palmitoyl-coenzyme A dehydrogenase, PtmO4, type-II acyl-CoA thioesterase, very long chain acyl-CoA dehydrogenase, very long-chain acyl-CoA dehydrogenase, very-long-chain acyl-CoA dehydrogenase, very-long-chain acyl-coenzyme A dehydrogenase, VLCAD, VLMCAD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.8 long-chain acyl-CoA dehydrogenase

Purification

Purification on EC 1.3.8.8 - long-chain acyl-CoA dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
35-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite
-
40-60% ammonium sulfate precipitation, DEAE-Sepharose, hydroxyapatite
-
40-70% ammonium sulfate precipitation, Sephadex G-150, DEAE-cellulose, preparative electrophoresis
-
40-75% ammonium sulfate precipitation, G-200 electrophoresis
-
40-80% ammonium sulfate precipitation, DEAE-Sephadex A-50, hydroxyapatite, matrex gel blue A, agarose-hexane-CoA, bio-gel A-0.5m
-
40-80% ammonium sulfate precipitation, Q-Sepharose I, Q-Sepharose II, hydroxyapatite
-
ammonium sulfate precipitation and DEAE-Sepharose Fast Flow column chromatography
-
ammonium sulfate precipitation, DEAE Sepharose column chromatography, and SP Sepharose column chromatography
-
ammonium sulfate precipitation, zone electrophoresis
-
DEAE-cellulose, blue dextran-sepharose, calcium phosphate gel-cellulose, blue dextran-sepharose, HPLC
-
DEAE-Sepharose Fast Flow column chromatography and SP-Sepharose Fast Flow column chromatography
-
Q-Sepharose, hydroxyapatite I, hydroxyapatite II
-
recombinant acdB-encoded enzyme from Escherichia coli
recombinant enzyme from Escherichia coli strain BL21(DE3)
-