1.3.8.7: medium-chain acyl-CoA dehydrogenase

This is an abbreviated version, for detailed information about medium-chain acyl-CoA dehydrogenase, go to the full flat file.

Reaction

a medium-chain acyl-CoA
+
electron-transfer flavoprotein
=
a medium-chain trans-2,3-dehydroacyl-CoA
+
reduced electron-transfer flavoprotein

Synonyms

ACAD-9, ACADM, ACD, acyl CoA dehydrogenase, acyl coenzyme A dehydrogenase, acyl dehydrogenase, acyl-CoA dehydrogenase, acyl-CoA dehydrogenase-9, dehydrogenase, acyl coenzyme A, EC 1.3.2.2, FadE, fatty acyl coenzyme A dehydrogenase, fatty-acyl-CoA dehydrogenase, general ACAD, general acyl CoA dehydrogenase, LCAD, LipB, MCAD, MCADH, medium chain acyl-CoA dehydrogenase, medium chain-specific acyl-CoA oxidase, medium-chain acyl CoA dehydrogenase, medium-chain acyl-CoA dehydrogenase, medium-chain acyl-coenzyme A dehydrogenase, medium-chain coenzyme A dehydrogenase, pMCAD, PP_1893, PP_2039, PP_2048, PP_2437

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.7 medium-chain acyl-CoA dehydrogenase

Temperature Stability

Temperature Stability on EC 1.3.8.7 - medium-chain acyl-CoA dehydrogenase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 37
-
biphasic inactivation, half-lives: 3 h, fast phase, and more than 700 h, slow phase, at 28°C and 2 h, fast phase, and approx. 230 h, slow phase, at 37°C for the wild-type enzyme, the corresponding values for the K304E mutant enzyme are 1.7 h, 100 h at 28°C and 1.25 h and 55 h at 37°C
25
-
mutant enzyme E376Q is instable at 25°C
37
-
60 min, bout 20% resudal activity
42
-
decreased stability of mutant enzymes Y42H and K304E above 42°C compared to wild-type enzyme
44.5
-
melting temperature, mutant Y158H
50
-
melting temperature, mutant R206C
52
-
melting temperature, mutant A52V; melting temperature, mutant R413S
52.5
-
melting temperature, mutant Y67H
53.5
-
melting temperature, mutant D266G; melting temperature, mutant R334K
54
-
melting temperature, mutant K329E
56.5
-
melting temperature, wild-type
60
-
11 min, reduced enzyme stable, oxidized enzyme unstable