1.3.8.4: isovaleryl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about isovaleryl-CoA dehydrogenase, go to the full flat file.

Word Map on EC 1.3.8.4

Reaction

isovaleryl-CoA
+
electron-transfer flavoprotein
=
3-methylcrotonyl-CoA
+
reduced electron-transfer flavoprotein

Synonyms

acyl-CoA dehydrogenase, i3VD, iso(3)valeryl-CoA dehydrogenase, isovaleric-CoA dehydrogenase, isovaleroyl-coenzyme A dehydrogenase, isovaleryl-coenzyme A dehydrogenase, IVD, IVDH, LiuA, SBCAD, short/branched chain acyl-CoA dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.4 isovaleryl-CoA dehydrogenase

Purification

Purification on EC 1.3.8.4 - isovaleryl-CoA dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by loading the supernatant from an induced culture onto an XK16/20 Fast Q fast flow column, overnight dialysis in potassium phosphate buffer, and hydroxyapatite column chromatography
-
of recombinant enzyme, using sonication, ammonium sulfate fractionation, column chromatography on DEAE-Sepharose and hydroxyapatite
-
partially from tuber, recombinant from Escherichia coli to over 90% purity
recombinant from Escherichia coli to over 90% purity
-
recombinant LiuA from Escherichia coli
-
using ammonium sulfate fractionation followed by DEAE-Sephadex A-50, hydroxyapatite, Matrex Gel Blue A, agarose-hexane-CoA, and Bio-Gel A-0.5 column chromatography
-
using auxin affinity chromatography matrix
-
using bacterial lysates through centrifugation, filtration through an Acrodisc filter, chromatography on a XK16/20 DEAE fast-flow column and hydroxyapatite resin column
-
using chromatography on Resource Q anion exchange column, BioRad CHT2 hydroxyapatite column and phenyl ether hydrophobic interaction column
-
using DEAE-Sepharose followed by hydroxyapatite column chromatography
-
using ion exchange column chromatography and isoelectric focusing
-
using sonication, ammonium sulfate treatment and column chromatography on DEAE-Sephadex A-50, hydroxylapatite, Matrex Gel Blue A and BioGel A-0.5 m
-
using sonication, ammonium sulfate treatment, column chromatography on DEAE-Sephadex A-50, hydroxylapatite and isoelectrofocusing
-