1.3.8.12: (2S)-methylsuccinyl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about (2S)-methylsuccinyl-CoA dehydrogenase, go to the full flat file.

Reaction

(2S)-methylsuccinyl-CoA
+
electron-transfer flavoprotein
=
2-methylfumaryl-CoA
+
reduced electron-transfer flavoprotein

Synonyms

MCD, methylsuccinyl-CoA dehydrogenase, PdMCD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.12 (2S)-methylsuccinyl-CoA dehydrogenase

Purification

Purification on EC 1.3.8.12 - (2S)-methylsuccinyl-CoA dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
recombinant His-tagged wild-tpye and mutant enzymes from Escherichia coli strain Rosetta pLys (DE3) by nickel affinity chromatography of cell-free supernatant, desalting gel filtration, tag cleavage by thrombin, gel filtration, and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography and ultrafiltration