1.3.8.12: (2S)-methylsuccinyl-CoA dehydrogenase

This is an abbreviated version!
For detailed information about (2S)-methylsuccinyl-CoA dehydrogenase, go to the full flat file.

Reaction

(2S)-methylsuccinyl-CoA
+
electron-transfer flavoprotein
=
2-methylfumaryl-CoA
+
reduced electron-transfer flavoprotein

Synonyms

MCD, methylsuccinyl-CoA dehydrogenase, PdMCD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.8 With a flavin as acceptor
                1.3.8.12 (2S)-methylsuccinyl-CoA dehydrogenase

Engineering

Engineering on EC 1.3.8.12 - (2S)-methylsuccinyl-CoA dehydrogenase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
T317G
site-directed mutagenesis, the mutant shows increased oxidase activity and reduced dehydrogenase activity compared to wild-type. The mutant directly reacts with O2
Y372I
site-directed mutagenesis, the mutant is inactive as oxidase
M375S
site-directed mutagenesis, the mutant is inactive as oxidase
Y378G
site-directed mutagenesis, the mutant is inactive as oxidase
E377N
site-directed mutagenesis, the mutant does not show increased oxidase activity although reduced dehydrogenase activity compared to wild-type
W315F
site-directed mutagenesis, the mutant does not show increased oxidase activity although reduced dehydrogenase activity compared to wild-type
W315F/T317G/E377N
site-directed mutagenesis, the mutant shows increased oxidase activity and reduced dehydrogenase activity compared to wild-type. The mutant directly reacts with O2
A282V
site-directed mutagenesis, the mutant shows increased activity with succinyl-CoA compare to wild-type
A282L
site-directed mutagenesis, inactive mutant
A282I
site-directed mutagenesis, inactive mutant
A282F
site-directed mutagenesis, inactive mutant
A282F/F284A
site-directed mutagenesis, mutant is inactive with succinyl-CoA, but shows residual activity with (S2)-methylsuccinyl-CoA
A282F/F284V
site-directed mutagenesis, inactive mutant
A282F/F284L
site-directed mutagenesis, inactive mutant
A282V
-
site-directed mutagenesis, the mutant shows increased activity with succinyl-CoA compare to wild-type
-
A282L
-
site-directed mutagenesis, inactive mutant
-
A282I
-
site-directed mutagenesis, inactive mutant
-
A282F
-
site-directed mutagenesis, inactive mutant
-
additional information