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1.3.7.12: red chlorophyll catabolite reductase

This is an abbreviated version!
For detailed information about red chlorophyll catabolite reductase, go to the full flat file.

Word Map on EC 1.3.7.12

Reaction

primary fluorescent chlorophyll catabolite
+ 2 oxidized ferredoxin [iron-sulfur] cluster =
red chlorophyll catabolite
 + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+

Synonyms

ACD2 protein, At-RCCR, AtRCCR, BoRCCR, BrRCCR, CaRCCR, EC 1.3.1.80, HvRCCR, PHAVU_008G280300g, RCC reductase, RCCR, RCCR-1, RCCR-2, red Chl catabolite reductase, red chlorophyll catabolite reductase, red-chlorophyll-catabolite reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.7 With an iron-sulfur protein as acceptor
                1.3.7.12 red chlorophyll catabolite reductase

Cloned

Cloned on EC 1.3.7.12 - red chlorophyll catabolite reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a partial gene sequence
chimeric RCCRs composed of portions of the Arabidopsis and the tomato proteins are expressed in Escherichia coli
cloning and expression in Escherichia coli
expression in Escherichia coli
-
expression of GST-tagged wild-type and F218V mutant RCCR lacking the chloroplast transit peptide, Met1 to Gln39
expression of wild-type and mutant enzymes lacking the chloroplast transit peptide (Met1 to Gln39) and N-termial residues 40-48 as GST-tagged enzymes
gene CaRCCR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree
gene RCCR, cloned from leaves, quantitative enzyme expression analysis
gene RCCR, quantitative RT-PCR enzyme expression analysis
gene RCCR, the enzyme interacts with the 7-hydroxymethyl chlorophyll a reductase, HCAR, in in yeast two-hybrid assay
overexpression of ACD2 protein makes the plants tolerant but not resistant to bacterial infection
-
recombinant expression of chimeric enzyme mutants in Escherichia coli
transcriptome analysis and real-time quantitative PCR enzyme expression analysis in wild-type and mutant lines