1.3.5.5: 15-cis-phytoene desaturase
This is an abbreviated version!
For detailed information about 15-cis-phytoene desaturase, go to the full flat file.
Word Map on EC 1.3.5.5
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1.3.5.5
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carotenoid
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herbicide
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zeta-carotene
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agriculture
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bleaching
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norflurazon
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synthesis
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phytofluene
- 1.3.5.5
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carotenoid
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herbicide
- zeta-carotene
- agriculture
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bleaching
- norflurazon
- synthesis
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phytofluene
Reaction
Synonyms
BoPDS, CitPDS1, CrtP, OsPDS, PDS, PDS1, PDS3, phytoene desaturase, plant-type phytoene desaturase
ECTree
1.3.5.5 15-cis-phytoene desaturaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 1.3.5.5 - 15-cis-phytoene desaturase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
15-cis-phytoene + 2 decyl-plastoquinone
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
15-cis-phytoene + 2 decyl-ubiquinone
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially
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-
?
15-cis-phytoene + 2 decylubiquinone
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
this quinone derivative is used in the study because the natural C45-ubiquinone is not available commercially
-
-
?
15-cis-phytoene + 2,5-dibromo-3-methyl-6-isopropyl-1,4-benzoquinone
9,15,9'-tricis-zeta-carotene + 2,5-dibromo-3-methyl-6-isopropyl-1,4-benzoquinol
-
-
-
-
?
15-cis-phytoene + 5-n-undecyl-6-hydroxy-4,7-dioxobenzothiazole + H2O
9,15,9'-tricis-zeta-carotene + 5-n-undecyl-4,6,7-trihydroxybenzothiazole
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-
-
-
?
15-cis-phytoene + duroquinone
9,15,9'-tricis-zeta-carotene + duroquinol
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-
-
-
?
15-cis-phytoene + plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
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the biosynthetic pathway of carotenoid synthesis involves several steps of desaturation requiring free energy, for instance the transition from phytoene to phytofluene achieved by phytoene desaturase. This reaction is associated with a reduction of plastoquinone. The oxidation of the plastoquinol is then achieved by a plastoquinol:oxygen oxidoreductase. Might additionally mediate the reduction of plastoquinone by internal reductants
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-
?
15,9'-dicis-phytofluene + plastoquinone
9,15,9'-tricis-zeta-carotene + plastoquinol
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-
-
-
?
15,9'-dicis-phytofluene + plastoquinone
9,15,9'-tricis-zeta-carotene + plastoquinol
-
-
-
?
15,9'-dicis-phytofluene + plastoquinone
9,15,9'-tricis-zeta-carotene + plastoquinol
Dunaliella salina CCAP 19/41 (PLY DF15)
-
-
-
?
15,9'-dicis-phytofluene + plastoquinone
9,15,9'-tricis-zeta-carotene + plastoquinol
-
-
-
?
15-cis-phytoene + 2 decyl-plastoquinone
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially. Decyl-plastoquinone is very effective as a cofactor of desaturation. The simultanous presence of NADP+ has no influence on the activity
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-
?
15-cis-phytoene + 2 decyl-plastoquinone
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
-
-
?
15-cis-phytoene + 2 decyl-plastoquinone
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
this quinone derivative is used in the study because the natural C45-plastoquinone is not available commercially
-
-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
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-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
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overall reaction
-
-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
overall reaction. The conversion rate with all-trans-phytoene is 10% of the value with the 15-cis isomer
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-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
the biosynthetic pathway of carotenoid synthesis involves several steps of desaturation requiring free energy, for instance the transition from phytoene to phytofluene achieved by phytoene desaturase. This reaction is associated with a reduction of plastoquinone. The oxidation of the plastoquinol is then achieved by a plastoquinol:oxygen oxidoreductase. Might additionally mediate the reduction of plastoquinone by internal reductants
-
-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
-
?
15-cis-phytoene + 2 plastoquinone
9,15,9'-tricis-zeta-carotene + 2 plastoquinol
-
-
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
phytoene desaturase is an enzyme of the carotenoid biosynthetic pathway
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
phytoene desaturase is an early enzyme of the carotenoid biosynthetic pathway
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
-
-
-
?
15-cis-phytoene + acceptor
9,15,9'-tricis-zeta-carotene + reduced acceptor
phytoene desaturase is an enzyme of the carotenoid biosynthetic pathway
-
-
?
15-cis-phytoene + plastoquinone
15,9'-dicis-phytofluene + plastoquinol
-
-
-
-
?
15-cis-phytoene + plastoquinone
15,9'-dicis-phytofluene + plastoquinol
-
-
-
?
15-cis-phytoene + plastoquinone
15,9'-dicis-phytofluene + plastoquinol
Dunaliella salina CCAP 19/41 (PLY DF15)
-
-
-
?
15-cis-phytoene + plastoquinone
15,9'-dicis-phytofluene + plastoquinol
-
-
-
?
15-cis-phytoene + plastoquinone
15,9'-dicis-phytofluene + plastoquinol
decyl-plastoquinone is used for enzyme assays
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-
?
additional information
?
-
geranylgeranyl diphosphate is catalysed by phytoene synthase and phytoene desaturase to phytoene and phytofluene, respectively. The subsequent steps involve desaturation, isomerisation and cyclisation reactions to form alpha- and beta-carotene stereoisomers, via all-trans lycopene
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-
-
additional information
?
-
Dunaliella salina CCAP 19/41 (PLY DF15)
geranylgeranyl diphosphate is catalysed by phytoene synthase and phytoene desaturase to phytoene and phytofluene, respectively. The subsequent steps involve desaturation, isomerisation and cyclisation reactions to form alpha- and beta-carotene stereoisomers, via all-trans lycopene
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-
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additional information
?
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benzoquinone cosubstrates are strongly preferred over naphthoquinones
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-
?
additional information
?
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PDS requires plastoquinone as a directly interacting co-substrate to reoxidize the enzyme-bound FADred formed upon desaturation. The enzyme structure has led to the conclusion that both lipophilic substrates are bound inside the same substrate cavity that cannot be occupied by both simultaneously. The central 15-cis configuration of phytoene mediates the regiospecificity in catalysis, overview. LC-MS analysis of PDS desaturation products produced from asymmetric (C35) 15-cis-norphytoene
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-
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additional information
?
-
-
the enzyme also shows activity with NADP+ as cosubstrate, the affinity for NADP+ is lower than for decylplastoquinone or decylubioquinone
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-
?
