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1.3.5.1: succinate dehydrogenase

This is an abbreviated version!
For detailed information about succinate dehydrogenase, go to the full flat file.

Word Map on EC 1.3.5.1

Reaction

succinate
+
a quinone
=
fumarate
 +
a quinol

Synonyms

8-methylmenaquinol:fumarate reductase, AaSdhB, bacterial succinate:quinone oxidoreductase flavoprotein, complex II, Complex II homolog, complex II of the respiratory chain, complex II succinate:ubiquinone oxidoreductase, DCPIP oxidoreductase, dehydrogenase, succinate, dehydrogenase/complex II, EC 1.3.5.4, EC 1.3.99.1, Fcc3, fdrB, FL cyt, Flavocytochrome c3, FRD, FrdA, FRdABCD, FrdC, FRdCAB, FrdD, fumarate reductase, fumarate reductase complex, fumaric hydrogenase, Ifc3, Iron(III)-induced flavocytochrome C3, iron-sulfur subunit of succinate dehydrogenase, menaquinol-1 fumarate reductase, menaquinol-fumarate oxidoreductase, menaquinol:fumarate oxidoreductase, methylmenaquinol:fumarate reductase, MFR, MFR complex, MfrA, MfrB, mitochondrial complex II, mitochondrial succinate dehydrogenase, mitochondrial succinate:ubiquinone oxidoreductase, More, mQFR, MSMEG_0416, MSMEG_0417, MSMEG_0418, MSMEG_0419, MSMEG_0420, MSMEG_1669, MSMEG_1670, MSMEG_1671, MSMEG_1672, non-classical succinate:quinone reductase, QFR, quinol-fumarate reductase, quinol:fumarate reductase, SDG, SDG-1, SDG-2, SDH, SDH1, SDH2, SDH2-1, SDH2-2, SDH3, SDH4, SdhA, sdhABE, SDHB, SdhC, sdhCAB, SdhCDAB, SdhD, SDISP, SQR, succinate dehydrogenase, succinate dehydrogenase (caldariellaquinone), succinate dehydrogenase (quinone), succinate dehydrogenase B, succinate dehydrogenase complex, succinate dehydrogenase flavoprotein subunit Sdh1p, succinate dehydrogenase iron-sulfur protein, succinate dehydrogenase iron-sulphur protein, succinate dehydrogenase subunit B, succinate oxidoreductase, succinate-2,6-dichlorophenolindophenol oxidoreductase, succinate-coenzyme Q reductase, succinate-quinone oxidoreductase, succinate-quinone reductase, succinate-ubiquinone oxidoreductase, succinate:caldariellaquinone oxidoreductase, succinate:menaquinone 7-reductase, succinate:menaquinone oxidoreductase, succinate:menaquinone reductase, succinate:MK reductase, succinate:quinone oxidoreductase, succinate:quinone reductase, succinate:quinone reductases, succinate:ubiquinone oxidoreductase, succinate:ubiquinone reductase, succinic acid dehydrogenase, succinic dehydrogenase, succinodehydrogenase, succinyl dehydrogenase, Tneu_0423

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.5 With a quinone or related compound as acceptor
                1.3.5.1 succinate dehydrogenase

Purification

Purification on EC 1.3.5.1 - succinate dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
a purification procedure is established to enrich the protein 24fold via a combination of anion exchange and gel filtration chromatography with a yield of 36% of the initial activity in the periplasm extract
-
and reconstitution into proteoliposomes
-
by selective resolution of complex II with chaotropic salts
-
comparison of methods
-
enzyme from membranes of strain 3G18, expressed from low copy plasmid
-
from complex II, i.e. EC 1.3.5.1
from EC 1.3.5.1
-
isolation from the soluble cytochrome b-c1 complex of the mitochondrial protein, which converts soluble succinate dehydrogenase into succinate-ubiquinone oxidoreductase using two different methods, method 1: treatment with Triton X-100 in the presence of urea, column chromatography on calcium phosphate and ammonium sulfate fractionation, method 2: ammonium acetate fractionation in the presence of deoxycholate, ammonium sulfate fractionation in the presence of urea, and differential centrifugation
-
native enzyme by ammonium sulfate fractionation and gel filtration followed by anion exchange chromatography
-
native enzyme complex from heart partially by ammonium sulfate fractionation
-
native enzyme from brain mitochondria by anion and cation exchange chromatography followed by heparin affinity chromatography
native enzyme from cell-free supernatant of cell extract by anion exchange chromatography, dialysis, and another anion exchange chromatography, followed gel filtration and ultrafiltration
Megalodesulfovibrio gigas
native enzyme from muscle mitochondria by anion exchange chromatography
of both succinate dehydrogenase and fumarate reductase using solubilization with Thesit and DEAE fast-flow chromatography
-
of larval and adult complex II, using DEAE-cellulofine column chromatography in the presence of 0.1% w/v sucrose monolaurate
-
partial, complex II preparation, using Triton X-100 extraction followed by ammonium sulfate precipitation
recombinant His10-tagged protein Sdh2D from Mycobacterium smegmatis mutant strain mc2 51 by nickel affinity chromatography, digitonin treatment, and gel filtration
A0QT08; A0QT07; A0QT10; A0QT09
recombinant His6- or His8-tagged enzyme from Thermus thermophilus by nickel affinity chromatography and gel filtration, untagged enzyme by anion exchange chromatography and gel filtration
-
recombinant non-tagged SdhA complxed with His6-tagged SdhE from Escherichia coli by nickel affinity chromatography and gel filtration to homogeneity, elution as a heterodimer with FAD covalently bound (to SdhA) within the binary complex
recombinant SQR
-
recombinant SQR, Q-Sepharose, Poros 50HQ, Sephacryl S-300
-
recombinant wild-type and mutant enzymes partially from strain DW35 by membrane preparation
-
soluble recombinant enzyme mutant E245Q from Escherichia coli strain DW35 by anion exchange chromatography, ultrafitration, a second different step of anion exchange chromatography, followed by gel filtration
subunit SdhE (formerly SdhC) expressed in Escherichia coli
Q97W79; Q97W78; Q97W77; Q97W76
succinate-quinone oxidoreductase is solubilized and purified using detergents 2.5% (w/v) sucrose monolaurate and 0.5% (w/v) Lubrol PX
-
succinate-ubiquinone reductase obtained from resolution of succinate-cytochrome c reductase using hydroxyapatite and ammonium sulfate fractionation
-
two forms, differing in protein charges
-
using ammonium sulfate fractionation, solvent extraction and deoxycholate-ammonium sulfate extraction
-
using cell homogenate, Triton X-100 treatment and chromatography on hydroxyapatite and DEAE-Sepharose column
-
using detergent solubilization, ammonium sulfate fractionation and gel filtration on Sephadex G-200
-
using solubilization with polyoxyethylene-9-lauryl ether and column chromatography on DEAE-Sepharose CL-6B
-
using solubilization with sucrose monolaurate and column chromatography on DEAE-cellulose
-
using solubilization with Thesit, column chromatography on HiLoad 26/10 Q-Sepharose and gel filtration on a Sephacryl S-300 column
-
using solubilization with Triton X-100 and column chromatography on DEAE and Ultrogel ACA
-
using solubilization with Triton X-100, chromatography on hydroxyapatite column and DEAE-Sephadex column, the enzyme elutes from the ion-exchange column in two forms, one containing and the other lacking cytochrome b
-
using treatment with detergent Thesit and chromatography on DEAE-Sepharose FF column, Poros 50HQ column and Sephacryl S-300 gel filtration column
-
using urea washing, Triton X-100 extraction, cholate-ammonium sulfate extraction, calcium phosphate gel and ammonium sulfate precipitation
-
wild-type enzyme by gel filtration to remove Triton X-100, and anion exchange chromatography, followed by hydroxylapatite chromatography, and again anion exchange chromatography and gel filtration
-