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1.3.3.6: acyl-CoA oxidase

This is an abbreviated version!
For detailed information about acyl-CoA oxidase, go to the full flat file.

Word Map on EC 1.3.3.6

Reaction

acyl-CoA
+
O2
=
trans-2,3-dehydroacyl-CoA
 +
H2O2

Synonyms

3alpha,7alpha, 12alpha-trihydroxy-5beta-cholestanoyl-CoA oxidase, ACO, ACO I, ACO-A1, ACO-I, ACO-II, ACOX, ACOX-1, ACOX-1.1, ACOX-1.2, ACOX-1.3, ACOX-2, ACOX-3, ACOX1, ACOX1-3I, ACOX1a, ACOX1b, ACOX1–3II, ACOX2, ACOX3, ACX, ACX1, ACX1.2, ACX2, ACX3, ACX4, ACX5, acyl coenzyme A oxidase, acyl-CoA dehydrogenase, Acyl-CoA oxidase, acyl-CoA oxidase 1, acyl-CoA oxidase 1a, acyl-CoA oxidase 1b, acyl-CoA oxidase 2, acyl-CoA oxidase 4, acyl-CoA oxidase I, acyl-CoA oxidase-1, acyl-CoA oxidase-II, acyl-CoA-oxidase, acyl-coenzyme A oxidase, acyl-coenzyme A oxidase 1, acyl-coenzyme A oxidase 4, acyl-coenzyme A: oxygen oxidoreductase, AOX, AOX1, AOX2, Aox2p, AOX3, Aox3p, AOX4, aoxA, Aoxp, AtACX1, AtACX2, AtACX3, AtSACX, BRCACox, CrACX2, Cre05.g232002, fatty acyl-CoA oxidase, fatty acyl-coenzyme A oxidase, long chain acyl-CoA oxidase, long chain fatty acyl coenzyme A oxidase, long chain fatty acyl-CoA oxidase, long-chain-specific acyl-CoA oxidase 1, MCAD Y375K, MCOX, oxidase, acyl-coenzyme A, palmitoyl-CoA oxidase, peroxisomal acyl-coenzyme A oxidase, Peroxisomal fatty acyl-CoA oxidase, POX1, Pox1p, POX4, PpACX1, Pristanoyl-CoA oxidase, SCOX, short chain-specific acyl-CoA oxidase, short-chain acyl-CoA oxidase, straight chain acyl-CoA oxidase, straight-chain acyl-coenzyme A oxidase, THCA-CoA oxidase, THCCox, Trihydroxycoprostanoyl-CoA oxidase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.6 acyl-CoA oxidase

Cloned

Cloned on EC 1.3.3.6 - acyl-CoA oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
ACO-I and ACO-II
-
ACOX-1, quantitative RT-PCR expression analysis, Comparison of ascaroside production and expression levels of acyl-CoA oxidase genes under different conditions, overview
ACOX-2, quantitative RT-PCR expression analysis, Comparison of ascaroside production and expression levels of acyl-CoA oxidase genes under different conditions, overview
ACOX-3, quantitative RT-PCR expression analysis, Comparison of ascaroside production and expression levels of acyl-CoA oxidase genes under different conditions, overview
cloned into a bacterial expression vector pLM1 with six continous His codons attached to the 5' end of the gene, overexpression of wild-type ands mutant enzymes E421D, E421A, E421Q and E421G in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli JM109 cells
expressed in Escherichia coli strains BL21 and C41, and in COS-7 cells (His-tagged enzyme)
-
expressed in in HEK293T cells and Saccharomyces cerevisiae
-
expressed in Saccharomyces cerevisiae
expression as His-tagged protein in Spodoptera frugiperda cells via infection with Baculovirus
-
expression in COS-7 cells
expression in Escherichia coli
expression in Escherichia coli as an active, N-terminal tagged His6 fusion protein
expression in Escherichia coli BL 21
expression in Escherichia coli of 2 genes: ACX1.1 and ACX1.2, sequence comparison with other species
-
expression in Escherichia coli of His-tagged proteins: AtSACX, AtACX1, and AtACX3, sequence analysis also with AtACX2
expression of AtACX1 and AtACX2 in Escherichia coli
-
expression of His-tagged enzyme in Escherichia coli
-
expression of human ACOX1b isoform in a mouse ACOX1b mutant can reverse the null phenotype, overview
-
gene ACX1, real-time quantitative PCR enzyme expression analysis in fruit from the different cultivars, recombinant expression of His-tagged enzyme in from vector pET32a in Escherichia coli
gene aoxA, seuence comparisons, recombinant expression from plasmid in Aspergillus nidulans strain MH11036, complementation of enzyme deficient aoxADELTA strain MH11074, expression as GFP-tagged enzyme showing PexE-dependent peroxisomal localisation
-
gene Cre05.g232002 or CrACX2, screening of a Chlamydomonas insertional mutant library identifies a strain strongly impaired in oil remobilization and defective in Cre05.g232002 (CrACX2), recombinant expression of CrACX2 in Escherichia coli. Overexpression of GFP-tagged enzyme in Chlamydomonas reinhardtii peroxisomes
genes POX1-POX6, heterologous co-expression of the different isozymes with polyhydroxyalkanoate synthase (phaC) of Pseudomonas aeruginosa in Yarrowia lipolytica strains, subcloning in Escherichia coli
-
His-tagged
-
into the pET24 vector and transformed into Escherichia coli BL21(DE3)
into vector pTZ18R
-
into vector pUTE300, expression in Escherichia coli JM109
overexpression in Escherichia coli
-
plant anti-sense constructs, investigation of substrate specificities and regulation
-
SCOX expression analysis in peroxisomal acyl-coenzyme A oxidase deficiency patients, overview
-
sequence analysis
sequence analysis and regulation
-
sequence analysis, expression analysis during development
-
transformed SK32 cells stably expressing one of the wild type Pex5p isoforms, Pex5pM and S restore the processing of Aox, but Pex5pL does not
-