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1.3.3.5: bilirubin oxidase

This is an abbreviated version!
For detailed information about bilirubin oxidase, go to the full flat file.

Word Map on EC 1.3.3.5

Reaction

2 bilirubin +

O2
= 2 biliverdin + 2 H2O

Synonyms

bilirubin oxidase, bilirubin oxidase M-1, bilirubin:oxygen oxidoreductase, blue Cu enzyme, BOD, BODx, BOX, BPUM_0542, copper oxidase, CotA, MCO, multicopper oxidase, MvBO, MvBOD, oxidase, bilirubin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.5 bilirubin oxidase

Specific Activity

Specific Activity on EC 1.3.3.5 - bilirubin oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00041
-
mutant enzyme M467L, using bilirubin as a substrate
0.00047
-
mutant enzyme N459A/M467F, using bilirubin as a substrate
0.00051
-
enzyme activity in small intestine
0.00064
-
mutant enzyme M467F, using bilirubin as a substrate
0.00157
-
enzyme activity in liver mitochondria
0.074
-
M467G mutant enzyme
0.3
-
ferrocyanide oxidase activity of mutant M467G
1.19
-
mutant enzyme M467Q, using bilirubin as a substrate
1.2
-
bilirubin oxidase activity of mutant D105A
1.67
-
mutant I402G
11
-
bilirubin oxidase activity of mutant D105E
15.45
-
purified wild type enzyme, at pH 7.0 and 37°C
214.3
-
purified enzyme after anion exchange chromatography step, pH 7.5, 25°C
25.4
-
purified enzyme with substrate 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid), pH 6.0, 24°C
30
-
authentic enzyme
46.1
-
recombinant wild type enzyme, using bilirubin as a substrate
5.5
-
mutant 1Ru-C457S
6.42
-
mutant 2Ru-C457S
8.03
-
enzyme immobilized on alginate-silicate beads
additional information