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1.3.2.3: L-galactonolactone dehydrogenase

This is an abbreviated version!
For detailed information about L-galactonolactone dehydrogenase, go to the full flat file.

Word Map on EC 1.3.2.3

Reaction

L-galactono-1,4-lactone
+
2 ferricytochrome c
=
L-ascorbate
+ 2 ferrocytochrome c + 2 H+

Synonyms

dehydrogenase, galactonolactone, ferricytochrome c oxidoreductase, galactono-1,4-lactone dehydrogenase, galactonolactone dehydrogenase, GALDH, GalL dehydrogenase, GalLDH, GLDase, GLDH, GLDH-A1, GLDH-B1, GLDH-D1, GLDH1, GLDHase, L-galactone-1,4-lactone dehydrogenase, L-galactono-1, 4-lactone dehydrogenase, L-galactono-1,4-lactone dehydrogenase, L-galactono-gamma-lactone dehydrogenase, L-GalLDH, L-GalLDH1, L-GalLDH2, LsL-GalLDH, RrGalLDH

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.2 With a cytochrome as acceptor
                1.3.2.3 L-galactonolactone dehydrogenase

Cloned

Cloned on EC 1.3.2.3 - L-galactonolactone dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding GLDH, subcloning in Escherichia coli strain HB101, and functional expression in transgenic Nicotiana tabacum leaves and roots via Agrobacterium tumefaciens LBA4404-mediated transformation under control of the CaMV 35S promoter. Despite the elevated GLDH activity, the L-ascorbate content in the leaves does not change in all lines, but increases 6-10fold in the roots of transgenic plants, overview
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in tobacco plants by Agrobacterium-mediated transformation under CaMV 35S constitutive promoter
expression in Escherichia coli
-
expression in Saccharomyces cerevisiae
for expression in Escherichia coli BL21DE3 cells
-
gene GDH-A1, from the nulli-tetrasomic lines of CS, chromosome 5A, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings
gene GDH-B1, from the nulli-tetrasomic lines of CS, chromosome 5B, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings
gene GDH-D1, from the nulli-tetrasomic lines of CS, chromosome 5D, exon and intron structure among TaGLDH-A1, -B1 and -D1 homologues, sequence comparisons, recombinant ectopic expression in transgenic Nicotiana benthamiana using the viral vector based on pea early browning virus (PEBV), the GLDH homologue confers increased GLDH activity when ectopically expressed in tobacco, quantitative and semi-quantitative RT-PCR expression analysis, functional recombinant expression of GFP-tagged enzyme from BSMV:GFP virus in seedlings
gene GLDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant 19fold overexpression in Lactuca sativa under the control of the pea plastocyanin promoter and via Agrobacterium tumefcáciens strain LBA4404 mediated transformation, semi-quantitative and quantitative real-time RT-PCR enzyme expression analysis. Transformation experiments in lettuce with gene under the control of the CaMV 35S promoter fails
gene GLDH, quantitative real-time RT-PCR enzyme expression analysis
gene GLDH1, DNA and amino acid sequence determination and analysis, quantitative real-time PCR enzyme expression analysis
gene L-GalLDH1, quantitative real-time PCR isozyme expression analysis
gene L-GalLDH2, quantitative real-time PCR isozyme expression analysis
GLDH sense and antisense are transformed into rice callus by Agrobacterium-mediated infection (strain EHA105)
-
into a pET-His6 vector for expression in Escherichia coli BL21DE3 cells
into pMAT037 and transformed into the EHA101 strain of Agrobacterium tumefaciens
overexpressed in lettuce Agrobacterium-mediated transformation
-