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1.3.1.97: botryococcene synthase

This is an abbreviated version!
For detailed information about botryococcene synthase, go to the full flat file.

Word Map on EC 1.3.1.97

Reaction

C30 botryococcene
+
NADP+
 +
diphosphate
=
presqualene diphosphate
 +
NADPH
 +
H+

Synonyms

botryococcene synthase, BSS, squalene synthase-like 3, squalene synthase-like protein 3, SSL-3

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.97 botryococcene synthase

Engineering

Engineering on EC 1.3.1.97 - botryococcene synthase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
N171A/G207Q
site-directed mutagenesis, combined N171A and G207Q mutations in the SSL-3 backbone result in complete conversion of SSL-3 from an enzyme that used presqualene diphosphate for botryococcene biosynthesis to one that used presqualene diphosphate for squalene biosynthesis
Y166A
site-directed mutagenesis, mutant substrate specificity and activity compared to the wild-type
Y166F
site-directed mutagenesis, mutant substrate specificity and activity compared to the wild-type
additional information
generation of an expression system for production of high levels of botryococcene in Nicotiana tabacum strain KY 1068 plants by diverting carbon flux from the cytosolic mevalonate pathway or the plastidic methylerythritol phosphate pathway by the targeted overexpression of an avian farnesyl diphosphate synthase along with botryococcene synthase SSL-3 and presqualene diphosphate synthase SSL-1, overview. The structure of purified botryococcene from tobacco is determined by 1H-NMR and 13C-NMR spectral analyses. Modifications of botryococcene in tobacco by coexpressed triterpene methyltransferases (TMTs) from Botryococcus braunii