1.3.1.91: tRNA-dihydrouridine20 synthase [NAD(P)+]

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For detailed information about tRNA-dihydrouridine20 synthase [NAD(P)+], go to the full flat file.

Reaction

Synonyms

dihydrouridine synthase, dihydrouridine synthase 2, dihydrouridine synthase C, Dus, DUS 2, DUS2, Dus2p, DusA, DusB, DusC, EcoDusC, hDUS2, HsDus2, SMM1, tRNA-dihydrouridine synthase 2, tRNA-dihydrouridine synthases

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.1 With NAD⁺ or NADP⁺ as acceptor

                1.3.1.91 tRNA-dihydrouridine20 synthase [NAD(P)+]

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Results	in table
12748	AA Sequence
3	Application
9	Cloned(Commentary)
13	Cofactor
5	Crystallization (Commentary)
24	Disease/ Diagnostics
1	Expression
12	General Information
1	KM Value [mM]
1	Localization
1	Metals/Ions
1	Molecular Weight [Da]
14	Natural Substrates/ Products (Substrates)
12	Organism
2	pH Optimum
5	Protein Variants
9	Purification (Commentary)
3	Reaction
12	Reference
3	Source Tissue
25	Substrates and Products (Substrate)
7	Subunits
28	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
1	Turnover Number [1/s]

Crystallization

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Crystallization on EC 1.3.1.91 - tRNA-dihydrouridine20 synthase [NAD(P)+]

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CRYSTALLIZATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

purified recombinant His-tagged and SeMet-labeled DusC, sitting drop vapour diffusion method, mixing of 200 nl of about 10 mg/ml of protein in 20 mM HEPES, pH 7.6, 1 mM MgCl2, 200 mM KCl, 7 mM 2-mercaptoethanol, and 10% glycerol, with 200 nl of reservoir solution containing 0.1 M Tris, pH 7.9, 0.2 M sodium acetate, and 12% PEG 4000 for the wild-type enzyme and 0.1 M imidazole, pH 8.0, 15% v/v 2-propanol, and 20% v/v glycerol for the SeMet-labeled enzyme, X-ray diffraction structure determination and analysis at 2.1 A resolution

Escherichia coli

P33371

743952

vapor diffusion method, using 1.6 M lithium sulfate and 100 mM HEPES pH 7.5

Escherichia coli

P32695

765481

mutant enzyme E294K, vapor diffusion method, using 30% (w/v) PEG 2000 MME, 200 mM ammonium sulfate, and 50 mM sodium acetate pH 5.5. Mutant enzyme E294K/Q305K, vapor diffusion method, using 30% (w/v) PEG 2000 MME, 200 mM ammonium sulfate, and 50 mM sodium acetate pH 5.0. Mutant enzyme Q305K, vapor diffusion method, using 2.2 M ammonium sulfate

Homo sapiens

Q9NX74

764155

purified recombinant catalytic HsDus2dusD domain and tRNA binding domain HsDus2dsRBD (Glu347-Lys451), by hanging drop vapor diffusion method, at 19°C, X-ray diffraction structure determination and crystal structure analysis, PDB IDs 4WFS and 4WFT

Homo sapiens

Q9NX74

745924

purified recombinant hDus2 catalytic and tRNA-recognition domains (residues 1-340), sitting drop vapour diffusion method, 300 nl of 10 mg/ml protein in 20 mM Tris, pH 8.0, 100 mM NaCl, 5 mM imidazole, and 5 mM DTT, are mixed with 0. 54 ml of reservoir solution containing 0.1 M MES-malic acid-Tris, pH 4.0, and 25% w/v PEG 1500, 2 days, 19°C, X-ray diffraction structure determination and analysis by single-wavelength anomalous diffraction at 1.9 A resolution, automated molecular replacement with different search models, and modeling

Homo sapiens

Q9NX74

743940