1.3.1.84: acrylyl-CoA reductase (NADPH)
This is an abbreviated version!
For detailed information about acrylyl-CoA reductase (NADPH), go to the full flat file.
Word Map on EC 1.3.1.84
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1.3.1.84
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propionyl-coa
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acrylate
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acuis
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3-hydroxypropionate
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3-hydroxypropionyl-coa
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dehydratase
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chloroflexus
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sedula
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crotonyl-coa
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aurantiacus
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ruegeria
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dimethylsulfoniopropionate
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metallosphaera
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3-hydroxypropionate/4-hydroxybutyrate
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hyper-sensitive
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pomeroyi
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lyases
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dmdas
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ethylmalonyl-coa
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sphaeroides
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roseobacters
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succinyl-coa
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synthesis
- 1.3.1.84
- propionyl-coa
- acrylate
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acuis
- 3-hydroxypropionate
- 3-hydroxypropionyl-coa
- dehydratase
- chloroflexus
- sedula
- crotonyl-coa
- aurantiacus
- ruegeria
- dimethylsulfoniopropionate
- metallosphaera
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3-hydroxypropionate/4-hydroxybutyrate
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hyper-sensitive
- pomeroyi
- lyases
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dmdas
- ethylmalonyl-coa
- sphaeroides
- roseobacters
- succinyl-coa
- synthesis
Reaction
Synonyms
Acr, acryloyl-CoA reductase, acryloyl-CoA reductase (NADPH), acryloyl-coenzyme A reductase, acrylyl-CoA reductase, acrylyl-coenzyme A reductase, AcuI, Msed_1426, NADPH-dependent acryloyl-CoA reductase activity, NADPH-dependent acrylyl-CoA reductase, RSP_1434, SPO1914, SPO_1914, yhdH
ECTree
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Engineering
Engineering on EC 1.3.1.84 - acrylyl-CoA reductase (NADPH)
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additional information
construction of mutant DELTAacuI::kan. The introduction of codon-optimized genes SPO_1914 or yhdH into the DELTAacuI::kan mutant of Rhodobacter sphaeroides on plasmid pMA5-1 complements 3-hydroxypropionate-dependent growth of the mutant strain
additional information
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construction of mutant DELTAacuI::kan. The introduction of codon-optimized genes SPO_1914 or yhdH into the DELTAacuI::kan mutant of Rhodobacter sphaeroides on plasmid pMA5-1 complements 3-hydroxypropionate-dependent growth of the mutant strain
additional information
metabolic engineering analysis of the the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle for improving the pathway flux, overview
additional information
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metabolic engineering analysis of the the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle for improving the pathway flux, overview
additional information
to construct an acrylic acid-producing pathway in Escherichia coli strain MG1655DELTArecADELTAendA(DE3)/pZL42, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) Metallosphaera sedula is accompanied by recombinant overexpression of succinyl-CoA synthetase (SCS) from Escherichia coli. The engineered strain produces 13.28 mg/l of acrylic acid. To construct a propionic acid-producing pathway, the same five genes are expressed, with the addition of Metallosphaera sedula acryloyl-CoA reductase (ACR). The engineered strain produces 1430 mg/l of propionic acid, detailed overview
additional information
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to construct an acrylic acid-producing pathway in Escherichia coli strain MG1655DELTArecADELTAendA(DE3)/pZL42, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) Metallosphaera sedula is accompanied by recombinant overexpression of succinyl-CoA synthetase (SCS) from Escherichia coli. The engineered strain produces 13.28 mg/l of acrylic acid. To construct a propionic acid-producing pathway, the same five genes are expressed, with the addition of Metallosphaera sedula acryloyl-CoA reductase (ACR). The engineered strain produces 1430 mg/l of propionic acid, detailed overview
additional information
-
to construct an acrylic acid-producing pathway in Escherichia coli strain MG1655DELTArecADELTAendA(DE3)/pZL42, heterologous expression of malonyl-CoA reductase (MCR), malonate semialdehyde reductase (MSR), 3-hydroxypropionyl-CoA synthetase (3HPCS), and 3-hydroxypropionyl-CoA dehydratase (3HPCD) Metallosphaera sedula is accompanied by recombinant overexpression of succinyl-CoA synthetase (SCS) from Escherichia coli. The engineered strain produces 13.28 mg/l of acrylic acid. To construct a propionic acid-producing pathway, the same five genes are expressed, with the addition of Metallosphaera sedula acryloyl-CoA reductase (ACR). The engineered strain produces 1430 mg/l of propionic acid, detailed overview
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additional information
-
metabolic engineering analysis of the the 3-hydroxypropionate/4-hydroxybutyrate (3HP/4HB) cycle for improving the pathway flux, overview
-
additional information
construction of a gene SPO1914 disruption mutant by homologous recombination of suicide plasmids, the mutant is unable to grow on acrylate as the sole carbon source