1.3.1.81: (+)-pulegone reductase

This is an abbreviated version!
For detailed information about (+)-pulegone reductase, go to the full flat file.

Word Map on EC 1.3.1.81

Reaction

(+)-isomenthone
+
NADP+
=
(+)-pulegone
+
NADPH
+
H+

Synonyms

(+)-pulegone reductase, More, pulegone reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.81 (+)-pulegone reductase

Purification

Purification on EC 1.3.1.81 - (+)-pulegone reductase

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PURIFICATION/commentary
ORGANISM
UNIPROT
LITERATURE
native enzyme partially by isolation of secretory cells from leaves
-
recombinant enzyme from Escherichia coli by anion exchange and hydrophobic interaction chromatography followed by SDS-PAGE and gel excision
-
recombinant enzyme from Escherichia coli strain BL21(DE3), native enzyme by oil gland secretory cell isolation procedure, two steps of anion exchange chromatography, hydroxyapatite chromatography, and beta-NADPH affinity chromatography, the activity is rapidly lost during purification on each matrix, 10fold purification accompanied by loss of over 98% of starting activity
-