1.3.1.77: anthocyanidin reductase [(2R,3R)-flavan-3-ol-forming]

This is an abbreviated version!
For detailed information about anthocyanidin reductase [(2R,3R)-flavan-3-ol-forming], go to the full flat file.

Word Map on EC 1.3.1.77

Reaction

a (2R,3R)-flavan-3-ol
+ 2 NAD(P)+ =
an anthocyanidin with a 3-hydroxy group
+ 2 NAD(P)H +
H+

Synonyms

AtANR, MtANR, ANR, anthocyanidin reductase, BAN, BANYULS, dihydroflavonol 4-reductase-like protein, BEN1, PtrANR1, CsANR, ANR1, ANR2, CsANR1, CsANR2, MdLAR1, FeANR, BpANR, RrANR, VbANR, GhANR1, anthocyanidin reductase 1, anthocyanidin reductase 2, ANR2a, ANRa, CssANRa, ANRb, CssANRb

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.77 anthocyanidin reductase [(2R,3R)-flavan-3-ol-forming]

Cloned

Cloned on EC 1.3.1.77 - anthocyanidin reductase [(2R,3R)-flavan-3-ol-forming]

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
constitutive expression of the enzyme under control of the cauliflower mosaic virus 35S promoter in Nicotiana tabacum and Arabidopsis. Tobacco lines expressing the enzyme from Medicago trunculata lose the pink flower pigmentation characteristics of wild-type and empty vector control plants
expressed in Escherichia coli
expressed in Escherichia coli strain BL21
-
expressed in Escherichia coli. Overexpressed in Nicotiana tabacum and Medicago truncatula for functional analysis
-
expressed in Escherichia coli. To investigate the function of PtrANR1, the open reading frame in sense or antisense orientation is introduced into Populus tomentosa Carr. plants for ectopic expression under the control of the cauliflower mosaic virus 35S promoter, respectively
-
expressed in Malus domestica via Agrobacterium tumefaciens-mediated transformation
-
expression in Escherichia coli
expression in Escherichia coli as a fusion protein with maltose-binding protein
expression in Saccharomyces cerevisiae
-
for expression in Escherichia coli cells
gene ANR, cloned from dormant bud-specific complementary DNA (cDNA) library, DNA and amino acid sequence analysis, sequence comparisons and phylogenetic analysis, semiquantitative RT-PCR expression analysis
gene anr, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, cloned from two cultivars, Hokkai T8 and T10, quantitative real-time RT-PCR enzyme expression analysis
gene ANR, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of His-Trx-tagged enzyme in Escherichia coli strain BL21(DE3), recombinant expression of GFP-tagged enzyme in Arabidopsis thaliana ban mutant
gene ANR1, co-overexpression with leucoanthocyanidin reductase (LAR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis
gene ANR1, quantitative real-time PCR enzyme expression analysis
gene ANR2, co-overexpression with leucoanthocyanidin reductase (LAR) in Nicotiana tabacum cv. Xanthi by Agrobacterium tumefaciens-mediated transformation, semiquantitative expression analysis
gene ANR2, quantitative real-time PCR enzyme expression analysis
gene ANRa, cloned from leaves, recombinant expression of His-tagged ANRa, recombinant overexpression in Nicotiana tabacum leaves leading to the formation of proanthocyanidins in flowers and the reduction of anthocyanins
gene ANRb, cloned from leaves, recombinant expression of His-tagged ANRa, recombinant overexpression in Nicotiana tabacum leaves leading to the formation of proanthocyanidins in flowers and the reduction of anthocyanins
gene BAN, DNA and amino acid sequence determination and analysis, sequence comparisons and genetic mapping, phylogenetic tree, overview
gene BAN, overexpression of the enzyme in Medicago truncatula hairy roots. Recombinant enzyme ANR does not generate epicatechin from epicatechin-cysteine conjugate in hairy roots
gene BpANR, sequence comparisons, quantitative RT-PCR expression analysis
-
gene FeANR, cloned from leaves, DNA and amino acid sequence determination and analysis, phylogenetic analysis
gene GhANR1, cloned from developing fibers, DNA and amino acid sequence determination and analysis, ectopic expression of GhANR11 in the Arabidopsis thaliana ban mutant, via transformation by Agrobacterium tumefaciens strain EHA 105, allows for the reconstruction of the ANR pathway and proanthocyanidin biosynthesis in the seed coat, recombinant expression of His-Trx-tagged enzyme in Escherichia coli strain BL21(DE3)
genes ANR1 and ANR2, quantitative real-time PCR enzyme expression analysis
genetic transformation of Arabidopsis thaliana with the Arabidopsis TT2 MYB transcription factor results in ectopic expression of the BANYULS gene, encoding anthocyanidin reductase, AHA10 encoding a P-type proton-pump and TT12 encoding a transporter involved in proanthocyanidin biosynthesis. When coupled with constitutive expression of PAP1, a positive regulator of anthocyanin biosynthesis, TT2 expression in Arabidopsis leads to the accumulation of proanthocyanidins, but only in a subset of cells in which the BANYULS promoter is naturally expressed. Ectopic expression of the maize Lc MYC transcription factor weakly induces AHA10 but does not induce BANYULS, TT12 or accumulation of proanthocyanidins
-
overexpression of gene ANR under control of the CaMV35S promoter via Agrobacterium tumefaciens strain EHA105 transformation, real-time quantitative PCR expression analysis, a number of genes encoding stress-responsivefunctional proteins are upregulated in the RrANR-overexpressing tobacco lines, phenotype, detailed overview. Rosa rugosa anthocyanidin reductase overexpression in Nicotiana tabacum enhances tobacco tolerance to abiotic stress through increased reactive oxygen species scavenging and modulation of abscisic acid signaling
recombinantly expressed in Escherichia coli
-