1.3.1.48: 13,14-dehydro-15-oxoprostaglandin 13-reductase

This is an abbreviated version!
For detailed information about 13,14-dehydro-15-oxoprostaglandin 13-reductase, go to the full flat file.

Word Map on EC 1.3.1.48

Reaction

11alpha-hydroxy-9,15-dioxoprostanoate
+
NAD(P)+
=
(13E)-11alpha-hydroxy-9,15-dioxoprost-13-enoate
+
NAD(P)H
+
H+

Synonyms

15-hydroxyprostaglandin dehydrogenase, 15-keto-PG reductase, 15-ketoprostaglandin DELTA-13-reductase, 15-ketoprostaglandin DELTA13-reductase, 15-ketoprostaglandinDELTA13-reductase, 15-oxoprostaglandin 13-oxidase, 15-oxoprostaglandin 13-reductase, 15-oxoprostaglandin DELTA13-reductase, 15-oxoprostaglandin-DELTA13-reductase, 15-PGDH, Ad-PGR, bifunctional leukotriene B4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase, bifunctional LTB4 12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase, DELTA13-15-ketoprostaglandin reductase, leukotriene B4 12-hydroxydehydrogenase/15-ketoprostaglandin 13-reductase, leukotriene B4 dehydrogenase, leukotriene B4-12-hydroxydehydrogenase/15-oxo-prostaglandin 13-reductase, LTB4 12-HD/PGR, LTBDH/PGR, NAD(P)H-dependent alkenal/one oxidoreductase, PG reductase, PGR, PGR-2, PGR1, prostaglandin 13-reductase, prostaglandin DELTA13-reductase, prostaglandin reductase 1, reductase, 15-oxo-DELTA13-prostaglandin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.48 13,14-dehydro-15-oxoprostaglandin 13-reductase

Specific Activity

Specific Activity on EC 1.3.1.48 - 13,14-dehydro-15-oxoprostaglandin 13-reductase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.00026
-
enzyme activity in cornea, substrate 15-ketoprostaglandin, cofactor NADPH
0.000389
-
enzyme activity in retina, substrate 15-ketoprostaglandin, cofactor NADPH
0.00085
-
enzyme activity in retinal pigment epithelium, substrate 15-ketoprostaglandin, cofactor NADPH
0.000945
-
enzyme purified from iris-ciliary cytosol
0.0012
-
enzyme activity in ciliary body, substrate 15-ketoprostaglandin, cofactor NADPH
0.00145
-
enzyme activity in iris, substrate 15-ketoprostaglandin, cofactor NADPH
0.0405
-
-
0.115
using 15-ketoprostaglandin E1 as a substrate, at 37C
0.1784
using 15-ketoprostaglandin E2 as a substrate, at 37C
0.2064
using 15-ketoprostaglandin F1alpha as a substrate, at 37C
0.2309
using 15-ketoprostaglandin F2alpha as a substrate, at 37C
0.393
-
-
0.425
-
-
2.56
-
-
2.6
-
recombinant enzyme expressed in Escherichia coli
additional information
after restoration of LTBDH/PGR expression in LTBDH/PGR-negative (H1299) or -low (A549) lung cancer cell lines, the restored enzyme induces apoptosis and growth inhibition in vitro, restoration of LTBDH/PGR expression is effective in preventing lung cancer growth in vitro and in vivo; cell cycle alterations induced by LTBDH/PGR over-expression are assessed with Ad-GFP or Ad-PGR after infection at 100 MOI for 72 h, after infection with Ad-PGR, the apoptosis population in A549 cells and H1299 cells is 8.59% and 9.57%, whereas apoptosis population is not detected significantly in H157 infected cells; effects of induced expression of LTBDH/PGR in H1299 cells, LTBDH/PGR expression increases in a dose and time dependent manner after tetracycline treatment, marked expression is observed at day 4 and significantly affects cell proliferation beginning day 5 causing reduction in cell number and suggesting that LTBDH/PGR expression inhibit growth of H1299 cells; the expression of apoptosis-related proteins BAX, Bcl-2 and CD44 in H1299 and H157 cells is not changed by LTBDH/PGR over-expression except for BAX which is decreased in H157 cells, endogenous p53 and p21 are not expressed in both H1299 and H157 cells