after restoration of LTBDH/PGR expression in LTBDH/PGR-negative (H1299) or -low (A549) lung cancer cell lines, the restored enzyme induces apoptosis and growth inhibition in vitro, restoration of LTBDH/PGR expression is effective in preventing lung cancer growth in vitro and in vivo
cell cycle alterations induced by LTBDH/PGR over-expression are assessed with Ad-GFP or Ad-PGR after infection at 100 MOI for 72 h, after infection with Ad-PGR, the apoptosis population in A549 cells and H1299 cells is 8.59% and 9.57%, whereas apoptosis population is not detected significantly in H157 infected cells
effects of induced expression of LTBDH/PGR in H1299 cells, LTBDH/PGR expression increases in a dose and time dependent manner after tetracycline treatment, marked expression is observed at day 4 and significantly affects cell proliferation beginning day 5 causing reduction in cell number and suggesting that LTBDH/PGR expression inhibit growth of H1299 cells
the expression of apoptosis-related proteins BAX, Bcl-2 and CD44 in H1299 and H157 cells is not changed by LTBDH/PGR over-expression except for BAX which is decreased in H157 cells, endogenous p53 and p21 are not expressed in both H1299 and H157 cells